通过高效液相色谱法从一名慢性肾衰竭患者的血液透析液中高效纯化人血浆β2-微球蛋白。

Efficient purification of human plasma beta 2-microglobulin from the haemodialysate of a patient with chronic renal failure by use of high-performance liquid chromatography.

作者信息

Chung T G, Saito A, Ogawa H, Moriyama A, Ohkubo I, Sasaki M, Takahashi T

出版信息

J Chromatogr. 1985 Apr 12;339(1):67-74. doi: 10.1016/s0378-4347(00)84628-9.

Abstract

Human plasma beta 2-microglobulin was isolated in a good yield (more than 80%) from the haemodialysate (blood ultrafiltrate) of a patient with chronic renal failure. The isolation procedure consisted of Sephadex G-100 gel filtration and two steps of high-performance liquid chromatography: reversed-phase high-performance liquid chromatography and gel permeation chromatography. The purified beta 2-microglobulin was homogeneous by sodium dodecyl sulphate polyacrylamide gel electrophoresis and two-dimensional electrophoresis.

摘要

从一名慢性肾衰竭患者的血液透析液（血液超滤物）中以较高产率（超过80%）分离出了人血浆β2-微球蛋白。分离过程包括Sephadex G - 100凝胶过滤以及两步高效液相色谱法：反相高效液相色谱法和凝胶渗透色谱法。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳和二维电泳分析，纯化后的β2-微球蛋白呈均一状态。