通过等电聚焦法对人β2-微球蛋白进行表征。
Characterization of human beta2-microglobulin by isoelectric focusing.
作者信息
Hall P W, Ricanati E S, Vacca C V
出版信息
Clin Chim Acta. 1977 May 16;77(1):37-42. doi: 10.1016/0009-8981(77)90399-0.
PMID:68840
Abstract
b2-Microglobulin (B2M) isolated from the urine of normal subjects and patients with cadaveric renal transplantation, showed 2 homologues by isoelectric focusing, one with a pI 5.3, the other with a pI 5.7. These proteins show identical molecular weights by dextran gel filtration and sodium dodecyl sulfate acrylamide gel electrophoresis. The immunogenic reactivity demonstrates partial identity using antiserum to human B2M from 2 different sources.
摘要
从正常受试者和尸体肾移植患者尿液中分离出的β2-微球蛋白（B2M），通过等电聚焦显示出2种同系物，一种的等电点为5.3，另一种的等电点为5.7。通过葡聚糖凝胶过滤和十二烷基硫酸钠聚丙烯酰胺凝胶电泳，这些蛋白质显示出相同的分子量。使用来自2种不同来源的抗人B2M抗血清，免疫反应性显示出部分同一性。
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