Wei Jingjie, Shi Yuqi, Wang Xiaofei, He Shunfu, Qi Xinzhang, Lu Rong, Gao Yuhang, Liu ZiChen, Wang Yingyun, Wu Yuanheng, Jin Yipeng
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Qinghai Tibet Plateau Wildlife Park, Xining, Qinghai, China.
Front Vet Sci. 2024 Aug 29;11:1418553. doi: 10.3389/fvets.2024.1418553. eCollection 2024.
In August 2021, an outbreak of Feline Panleukopenia Virus (FPV) was observed in four 3-month-old Pallas' cats at Xining Wildlife Park. Despite timely intervention, the Pallas'cat cubs continued to experience clinical symptoms including diarrhea, seizures, and decreased white blood cell count, and all four cats died.
FPV clinical suspicions were initially confirmed by positive Polymerase Chain Reaction (PCR) testing. Pathological and immunohistochemical examinations (IHC) were performed on some organs, and the results showed that, encephalitis, viral enteritis, and splenitis occurred.
The virus replicates extensively in the cytoplasm of lymphocytes and macrophages in the lamina propria of the small intestine mucosa. A strain of FPV was successfully isolated and culture in CRFK cells. Through molecular identification, sequence analysis, and phylogenetic analysis of the VP2 gene in this strain, we have revealed the presence of a novel synonymous mutation. From July to December 2021, surveillance on stray cats and susceptible wildlife at Xining Wildlife Park indicated widespread FPV transmission.
The findings highlight the urgent need for ongoing epidemiological monitoring and active disinfection measures to prevent FPV transmission in wildlife parks.
2021年8月，西宁野生动物园的4只3个月大的兔狲幼崽中观察到猫泛白细胞减少症病毒（FPV）爆发。尽管进行了及时干预，但兔狲幼崽仍持续出现腹泻、癫痫发作和白细胞计数下降等临床症状，4只猫全部死亡。
最初通过聚合酶链反应（PCR）检测呈阳性确诊了FPV临床疑似病例。对部分器官进行了病理和免疫组化检查（IHC），结果显示发生了脑炎、病毒性肠炎和脾炎。
该病毒在小肠黏膜固有层的淋巴细胞和巨噬细胞胞质中广泛复制。在CRFK细胞中成功分离培养出一株FPV。通过对该毒株VP2基因进行分子鉴定、序列分析和系统发育分析，发现了一个新的同义突变。2021年7月至12月，对西宁野生动物园的流浪猫和易感野生动物进行监测，结果表明FPV传播广泛。
研究结果凸显了持续进行流行病学监测和采取积极消毒措施以预防野生动物公园内FPV传播的迫切需求。
