孟加拉国猫泛白细胞减少症病毒 VP2 基因的首次分子特征和系统进化分析。
First molecular characterization and phylogenetic analysis of the VP2 gene of feline panleukopenia virus in Bangladesh.
机构信息
Department of Medicine, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh.
Department of Comparative Biomedical Sciences, College of Veterinary Medicine, Mississippi State University, Starkville, MS, USA.
出版信息
Arch Virol. 2021 Aug;166(8):2273-2278. doi: 10.1007/s00705-021-05113-y. Epub 2021 May 31.
Feline panleukopenia virus (FPV) is a highly contagious infectious pathogen of cats globally. However, there is no information on the molecular identification and characterization of FPV in Bangladesh. Here, 8.16% (8/98) and 18.37% (18/98) of diarrheic cats tested positive for FPV by an immunochromatography (IC) test and PCR, respectively. The IC test showed 44.44% sensitivity and 100% specificity in comparison with PCR. Our newly sequenced Bangladeshi FPV strain (MN826076) showed the highest (99.71%) sequence identity to strains from the United Arab Emirates (UAE). Strain MN826076 contained two characteristic amino acid variations in VP2 identifying it as an FPV strain: valine at position 103 and aspartic acid at position 323. Phylogenetically, the VP2 of strain MN826076 was found to be closely related to 19 FPV strains, sharing the same clade.
猫泛白细胞减少症病毒（FPV）是一种在全球范围内高度传染的猫科动物感染病原体。然而，在孟加拉国，尚无关于 FPV 的分子鉴定和特征描述的信息。在此，通过免疫层析（IC）试验和 PCR 分别检测到 8.16%（8/98）和 18.37%（18/98）的腹泻猫呈 FPV 阳性。与 PCR 相比，IC 试验的灵敏度为 44.44%，特异性为 100%。我们新测序的孟加拉国 FPV 株（MN826076）与来自阿拉伯联合酋长国（UAE）的毒株具有最高的（99.71%）序列同一性。MN826076 株在 VP2 中含有两个特征性的氨基酸变异，可将其鉴定为 FPV 株：第 103 位的缬氨酸和第 323 位的天冬氨酸。系统进化分析表明，MN826076 株的 VP2 与 19 株 FPV 株密切相关，属于同一进化枝。
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