promotes transcriptional activation and inhibits ferroptosis of CRC cells through p300/H3K27ac.

This study investigated the role of lncRNA in ferroptosis of colorectal cancer (CRC). Real time quantitative polymerase chain reaction or western blot experiments were performed to examine relevant mRNAs and proteins expression. The kit assays evaluated malondialdehyde, iron, Fe and glutathione levels. ROS levels were verified by flow cytometry. Chromatin immunoprecipitation and RNA immunoprecipitation analysis monitored the correlation among , H3K27ac, p300 and ARNTL2. or knockdown facilitated erastin-induced ferroptosis. The interaction between and p300 resulted in the enhancement of H3K27ac levels at promoter to promote transcriptional activity. overexpression reversed the promoting effect of knockdown on ferroptosis. inhibited the ferroptosis in CRC by epigenetically upregulating the transcriptional activity of .