General surgery Departmet, Zhongshan Hospital (Xiamen), Fudan University & Xiamen Clinical Research Center for Cancer Therapy, Xiamen, Fujian Province, 351015, P.R. China.
Epigenomics. 2024;16(15-16):1097-1115. doi: 10.1080/17501911.2024.2387528. Epub 2024 Sep 13.
This study investigated the role of lncRNA in ferroptosis of colorectal cancer (CRC). Real time quantitative polymerase chain reaction or western blot experiments were performed to examine relevant mRNAs and proteins expression. The kit assays evaluated malondialdehyde, iron, Fe and glutathione levels. ROS levels were verified by flow cytometry. Chromatin immunoprecipitation and RNA immunoprecipitation analysis monitored the correlation among , H3K27ac, p300 and ARNTL2. or knockdown facilitated erastin-induced ferroptosis. The interaction between and p300 resulted in the enhancement of H3K27ac levels at promoter to promote transcriptional activity. overexpression reversed the promoting effect of knockdown on ferroptosis. inhibited the ferroptosis in CRC by epigenetically upregulating the transcriptional activity of .
本研究探讨了长链非编码 RNA 在结直肠癌（CRC）铁死亡中的作用。实时定量聚合酶链反应或 Western blot 实验检测相关 mRNA 和蛋白表达。试剂盒检测评估丙二醛、铁、Fe 和谷胱甘肽水平。通过流式细胞术验证 ROS 水平。染色质免疫沉淀和 RNA 免疫沉淀分析监测之间的相关性 ，H3K27ac，p300 和 ARNTL2。或 敲低促进 erastin 诱导的铁死亡。与 p300 的相互作用导致 启动子处 H3K27ac 水平升高，从而促进 转录活性。过表达逆转了 敲低对铁死亡的促进作用。通过表观遗传地上调 的转录活性抑制 CRC 中的铁死亡。
