Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China, Jinan University, Guangzhou 510632, China.
Kekulé-Institute for Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagk-Straße 1, 53121 Bonn, Germany.
Org Biomol Chem. 2024 Oct 9;22(39):7971-7975. doi: 10.1039/d4ob01348d.
The cyclisation mechanism of the fungal fusicoccane (FC)-type diterpene synthase (DTS) TadA was investigated by extensive isotopic labelling experiments, and the pH-dependency of the product selectivity of this enzyme was explored. These studies provide new insights into the cyclisation mechanisms of FC-type DTSs.
通过广泛的同位素标记实验研究了真菌 Fusicoccane（FC）型二萜合酶（DTS）TadA 的环化机制，并探讨了该酶产物选择性的 pH 依赖性。这些研究为 FC 型 DTS 的环化机制提供了新的见解。
