Kekulé-Institute for Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagk-Strasse 1, 53121, Bonn, Germany.
Angew Chem Int Ed Engl. 2019 Jul 1;58(27):9230-9233. doi: 10.1002/anie.201902950. Epub 2019 May 24.
Three diterpene synthases from actinomycetes have been studied. The first enzyme from Streptomyces cattleya produced the novel compound cattleyene. The other two enzymes from Nocardia testacea and Nocardia rhamnosiphila were identified as phomopsene synthases. The cyclisation mechanism of cattleyene synthase and the EIMS fragmentation mechanism of its product were extensively studied by incubation experiments with isotopically labelled precursors. Oxidative transformations expanded the chemical space of these unique diterpenes.
已经研究了三种来自放线菌的二萜合酶。第一种来自卡特利链霉菌的酶产生了新型化合物卡特利烯。另外两种来自红色诺卡氏菌和雷氏糖多孢菌的酶被鉴定为胡椒烯合酶。通过用同位素标记的前体进行孵育实验，广泛研究了卡特利烯合酶的环化机制及其产物的 EIMS 碎裂机制。氧化转化扩展了这些独特二萜的化学空间。
