Kunitake S T, La Sala K J, Kane J P
J Lipid Res. 1985 May;26(5):549-55.
Among the apoA-I-containing lipoproteins isolated by selected-affinity immunosorption from human serum and plasma, we have identified a subpopulation which, unlike the bulk of high density lipoproteins, has pre-beta electrophoretic mobility. This pre-beta subpopulation can be observed directly in fresh plasma by immunoelectrophoresis. It contains phospholipid and free and esterified cholesterol, but protein constitutes 90% of its mass. Apolipoprotein A-I is the predominant apolipoprotein in this subpopulation; apolipoprotein A-II and the B lipoproteins are not detected. The protein moiety of this subpopulation exhibits markedly lower helicity than that of high density lipoproteins isolated by ultracentrifugation.
在通过选择性亲和免疫吸附从人血清和血浆中分离出的含载脂蛋白A-I的脂蛋白中，我们鉴定出了一个亚群，该亚群与大多数高密度脂蛋白不同，具有前β电泳迁移率。通过免疫电泳可直接在新鲜血浆中观察到这个前β亚群。它含有磷脂、游离胆固醇和酯化胆固醇，但蛋白质占其质量的90%。载脂蛋白A-I是这个亚群中的主要载脂蛋白；未检测到载脂蛋白A-II和B类脂蛋白。这个亚群的蛋白质部分的螺旋度明显低于通过超速离心分离出的高密度脂蛋白。
