Department of Pharmaceutical Sciences, College of Pharmacy, Larkin University, Miami, FL, 33169, USA.
Eur J Drug Metab Pharmacokinet. 2024 Nov;49(6):661-676. doi: 10.1007/s13318-024-00912-5. Epub 2024 Sep 13.
Cytochrome P450 enzymes (CYPs) represent a diverse family of heme-thiolate proteins involved in the metabolism of a wide range of endogenous compounds and xenobiotics. In recent years, proteomics and metabolomics have been used to obtain a comprehensive insight into the role of CYPs in health and disease aspects. The objective of the present work is to better understand the status of proteomics and metabolomics in CYP research in optimizing therapeutics and patient safety from a personalized medicine approach. The literature used in this narrative review was procured by electronic search of PubMed, Medline, Embase, and Google Scholar databases. The following keywords were used in combination to identify related literature: "proteomics," "metabolomics," "cytochrome P450," "drug metabolism," "disease conditions," "proteome," "liquid chromatography-mass spectrometry," "integration," "metabolites," "pathological conditions." We reviewed studies that utilized proteomics and metabolomics approaches to explore the multifaceted roles of CYPs in identifying disease markers and determining the contribution of CYP enzymes in developing treatment strategies. The applications of various cutting-edge analytical techniques, including liquid chromatography-mass spectrometry, nuclear magnetic resonance, and bioinformatics analyses in CYP proteomics and metabolomics studies, have been highlighted. The identification of CYP enzymes through metabolomics and/or proteomics in various disease conditions provides key information in the diagnostic and therapeutic landscape. Leveraging both proteomics and metabolomics presents a powerful approach for an exhaustive exploration of the multifaceted roles played by CYP enzymes in personalized medicine. Proteomics and metabolomics have enabled researchers to unravel the complex connection between CYP enzymes and metabolic markers associated with specific diseases. As technology and methodologies evolve, an integrated approach promises to further elucidate the role of CYPs in human health and disease, potentially ushering in a new era of personalized medicine.
细胞色素 P450 酶（CYPs）是一类广泛参与内源性化合物和外源性物质代谢的血红素硫醇蛋白家族。近年来，蛋白质组学和代谢组学已被用于深入了解 CYP 在健康和疾病方面的作用。本研究旨在从个性化医疗的角度更好地了解蛋白质组学和代谢组学在 CYP 研究中优化治疗和患者安全的现状。本综述中使用的文献是通过电子搜索 PubMed、Medline、Embase 和 Google Scholar 数据库获得的。使用以下关键词组合来识别相关文献：“蛋白质组学”、“代谢组学”、“细胞色素 P450”、“药物代谢”、“疾病状况”、“蛋白质组”、“液相色谱-质谱联用”、“整合”、“代谢物”、“病理状况”。我们综述了利用蛋白质组学和代谢组学方法探索 CYP 在识别疾病标志物和确定 CYP 酶在制定治疗策略中的贡献的多方面作用的研究。强调了各种前沿分析技术在 CYP 蛋白质组学和代谢组学研究中的应用，包括液相色谱-质谱联用、核磁共振和生物信息学分析。通过代谢组学和/或蛋白质组学在各种疾病条件下鉴定 CYP 酶提供了在诊断和治疗领域的关键信息。利用蛋白质组学和代谢组学可以全面探索 CYP 酶在个性化医疗中的多方面作用。蛋白质组学和代谢组学使研究人员能够揭示 CYP 酶与特定疾病相关的代谢标志物之间的复杂联系。随着技术和方法的不断发展，综合方法有望进一步阐明 CYP 在人类健康和疾病中的作用，可能开创个性化医疗的新时代。
