Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Sciences, China Agricultural University, Beijing 100193, China.
National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, China.
J Agric Food Chem. 2024 Sep 25;72(38):20862-20871. doi: 10.1021/acs.jafc.4c05055. Epub 2024 Sep 13.
The role of insect UDP-glycosyltransferases (UGTs) in the detoxification of insecticides has rarely been reported. A UGT gene was previously found overexpressed in a fenvalerate-resistant strain of . Herein, was cloned, and its involvement in insecticide detoxification was investigated. was highly expressed in the larvae, mainly in the fat body and midgut. Treatment with UGT inhibitors 5-nitrouracil and sulfinpyrazone significantly enhanced the fenvalerate toxicity. Knocking down by RNAi significantly increased the larvae mortality by 17.89%. was further knocked out by CRISPR/Cas9, and a homozygous strain (HD-d) with a C-base deletion at exon 2 was obtained. The sensitivity of HD-d to fenvalerate, deltamethrin, cyantraniliprole, acetamiprid, and lufenuron increased significantly, with sensitivity index increased 2.523-, 2.544-, 2.250-, 2.473-, and 3.556-fold, respectively. These results suggested that was involved in the detoxification of to insecticides mentioned above, shedding light upon further understanding of the detoxification mechanisms of insecticides by insect UGTs.
昆虫 UDP-糖基转移酶 (UGTs) 在杀虫剂解毒中的作用很少被报道。先前在氰戊菊酯抗性品系 中发现了一个过度表达的 UGT 基因。在此，克隆了 ，并研究了其在杀虫剂解毒中的作用。 在幼虫中高度表达，主要在脂肪体和中肠中表达。用 UGT 抑制剂 5-硝基尿嘧啶和磺吡唑酮处理可显著增强氰戊菊酯的毒性。通过 RNAi 敲低 可使幼虫死亡率显著增加 17.89%。 进一步通过 CRISPR/Cas9 敲除，获得了一个在第 2 外显子处有 C 碱基缺失的纯合株系 (HD-d)。HD-d 对氰戊菊酯、溴氰虫酰胺、虫螨腈、噻虫胺和灭蝇脲的敏感性显著增加，敏感性指数分别增加了 2.523 倍、2.544 倍、2.250 倍、2.473 倍和 3.556 倍。这些结果表明 参与了上述杀虫剂对 的解毒，为进一步了解昆虫 UGTs 对杀虫剂的解毒机制提供了依据。
