Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China; Department of Entomology, China Agricultural University, Beijing 100193, China.
Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.
Pestic Biochem Physiol. 2024 Jun;202:105918. doi: 10.1016/j.pestbp.2024.105918. Epub 2024 Apr 20.
Transcription factors play an important role in regulating the expression of detoxification genes (e.g. P450s) that confer insecticide resistance. Our previous study identified a series of candidate transcription factors (CYP6B7-fenvalerate association proteins, CAPs) that may be related to fenvalerate-induced expression of CYP6B7 in a field HDTJ strain of H. armigera. Whether these CAPs can mediate the transcript of CYP6B7 induced by fenvalerate in a susceptible HDS strain of H. armigera remains unknown. Further study showed that the expression levels of multiple CAPs were significantly induced by fenvalerate in HDS strain. Knockdown of CAP19 [fatty acid synthase-like (FAS)], CAP22 [polysaccharide biosynthesis domain-containing protein 1 (PBDC1)], CAP24 [5-formyltetrahydrofolate cycloligase (5-FCL)], CAP30 [peptidoglycan recognition protein LB-like (PGRP)] and CAP33 [NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (NDUFA11)] resulted in significant inhibition of CYP6B7 and some other P450 genes expression; meanwhile, the sensitivity of HDS strain larvae to fenvalerate was significantly increased. In addition, PBDC1, PGRP and NDUFA11, either alone or in combination, could significantly enhance the activity of CYP6B7 promoter in HDS strain, as well as the expression level of CYP6B7 gene in Sf9 cells line. These results suggested that PBDC1, PGRP and NDUFA11 may be involved in the transcript regulation of key detoxifying genes in response to fenvalerate in HDS strain of H. armigera.
转录因子在调节解毒基因（如 P450s）的表达中发挥重要作用，这些基因赋予昆虫对杀虫剂的抗性。我们之前的研究鉴定了一系列候选转录因子（CYP6B7-氰戊菊酯相关蛋白，CAPs），它们可能与棉铃虫田间 HDTJ 品系中 CYP6B7 被氰戊菊酯诱导表达有关。这些 CAPs 是否能介导对氰戊菊酯敏感的 HDS 品系棉铃虫中 CYP6B7 的转录，目前尚不清楚。进一步的研究表明，多种 CAPs 的表达水平在 HDS 品系中被氰戊菊酯显著诱导。CAP19[脂肪酸合酶样（FAS）]、CAP22[多糖生物合成结构域蛋白 1（PBDC1）]、CAP24[5-甲酰四氢叶酸环化酶（5-FCL）]、CAP30[肽聚糖识别蛋白 LB 样（PGRP）]和 CAP33[NADH 脱氢酶[泛醌]1 亚基 11（NDUFA11）]的敲低导致 CYP6B7 和其他一些 P450 基因的表达显著抑制；同时，HDS 品系幼虫对氰戊菊酯的敏感性显著增加。此外，PBDC1、PGRP 和 NDUFA11 单独或联合使用，均可显著增强 HDS 品系中 CYP6B7 启动子的活性，以及 Sf9 细胞系中 CYP6B7 基因的表达水平。这些结果表明，PBDC1、PGRP 和 NDUFA11 可能参与了 HDS 品系棉铃虫对氰戊菊酯的关键解毒基因转录调控。
