Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.
Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.
Pestic Biochem Physiol. 2024 Feb;199:105775. doi: 10.1016/j.pestbp.2024.105775. Epub 2024 Jan 11.
Insect cuticular protein (ICP) plays an important role in insect growth and development. However, research on the role of ICP in insecticide resistance is very limited. In this study, insect cuticular protein genes LCP17 and SgAbd5 were cloned and characterized in Helicoverpa armigera based on previous transcriptome data. The functions of LCP17 and SgAbd5 genes in fenvalerate resistance were assessed by RNA interference (RNAi), and their response to fenvalerate was further detected. The results showed that LCP17 and SgAbd5 were overexpressed in the fenvalerate-resistant strain comparing with a susceptible strain. The open reading frames of LCP17 and SgAbd5 genes were 423 bp and 369 bp, encoding 141 and 123 amino acids, respectively. LCP17 and SgAbd5 genes were highly expressed in the larval stage, but less expressed in the adult and pupal stages. The expression level of LCP17 and SgAbd5 genes increased significantly after fenvalerate treatment at 24 h. When the cotton bollworms larvae were exposed to fenvalerate at LD level, RNAi-mediated silencing of LCP17 and SgAbd5 genes increased the mortality from 50.68% to 68.67% and 63.89%, respectively; the mortality increased to even higher level, which was 73.61%, when these two genes were co-silenced. Moreover, silencing of these two genes caused the cuticle lamellar structure to become loose, which led to increased penetration of fenvalerate into the larvae. The results suggested that LCP17 and SgAbd5 may be involved in the resistance of cotton bollworm to fenvalerate, and LCP17 and SgAbd5 could serve as potential targets for H. armigera control.
昆虫表皮蛋白（ICP）在昆虫的生长发育中起着重要作用。然而，关于 ICP 在杀虫剂抗性中的作用的研究非常有限。在本研究中，基于先前的转录组数据，克隆并鉴定了棉铃虫中的昆虫表皮蛋白基因 LCP17 和 SgAbd5。通过 RNA 干扰（RNAi）评估了 LCP17 和 SgAbd5 基因在氰戊菊酯抗性中的作用，并进一步检测了它们对氰戊菊酯的反应。结果表明，与敏感品系相比，LCP17 和 SgAbd5 在氰戊菊酯抗性品系中过度表达。LCP17 和 SgAbd5 基因的开放阅读框分别为 423bp 和 369bp，分别编码 141 和 123 个氨基酸。LCP17 和 SgAbd5 基因在幼虫期高度表达，但在成虫和蛹期表达较少。LCP17 和 SgAbd5 基因在氰戊菊酯处理 24 小时后表达水平显著增加。当棉铃虫幼虫暴露在 LD 水平的氰戊菊酯下时，LCP17 和 SgAbd5 基因的 RNAi 介导沉默分别将死亡率从 50.68%提高到 68.67%和 63.89%；当这两个基因同时沉默时，死亡率甚至更高，达到 73.61%。此外，这些基因的沉默导致表皮层结构变得松散，这导致氰戊菊酯更容易渗透到幼虫体内。结果表明，LCP17 和 SgAbd5 可能参与了棉铃虫对氰戊菊酯的抗性，LCP17 和 SgAbd5 可以作为棉铃虫防治的潜在靶标。
