通过选择性酶促乙醇解富集甘油酯中的二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）
Enrichment of EPA and DHA in glycerides by selective enzymatic ethanolysis.
作者信息
Yu Junwen, Fu Yijie, Tang Xiao, Bao Weijia, Li Zongrun, Wang Xiaowen, Wang Xiaosan
机构信息
Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, National Engineering Research Center for Functional Food, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China.
Ministerial and Provincial Co-Innovation Centre for Endemic Crops Production with High-quality and Effciency in Loess Plateau, Shanxi Agricultural University, Taigu, Taiyuan, Shanxi, 030801, PR China.
出版信息
Food Chem. 2025 Jan 15;463(Pt 2):141226. doi: 10.1016/j.foodchem.2024.141226. Epub 2024 Sep 10.
It has been reported that eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in glycerides have various biological functions. This study presents an effective method for enriching glycerides rich in EPA and DHA through lipase-catalyzed alcoholysis. The results showed that Eversa® Transform 2.0 had the strongest discrimination against DHA and EPA in alcoholysis, which was verified by molecular docking. Additionally, selectivity of the lipase and ratio of DHA and EPA in glyceride products were significantly affected by alcohol type. Under the optimal conditions, the contents of EPA and DHA in glycerides after ethanolysis reached 12.91 % and 55.40 %, respectively, with a DHA yield of 79.22 %. In this study, an interesting finding was that Eversa® Transform 2.0 could effectively differentiate EPA and DHA during alcoholysis to allow us to prepare DHA-enriched glycerides and EPA-enriched ethyl esters after removing saturated and monounsaturated ethyl esters.
据报道，甘油酯中的二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）具有多种生物学功能。本研究提出了一种通过脂肪酶催化醇解来富集富含EPA和DHA的甘油酯的有效方法。结果表明，Eversa® Transform 2.0在醇解过程中对DHA和EPA的区分能力最强，这通过分子对接得到了验证。此外，脂肪酶的选择性以及甘油酯产物中DHA和EPA的比例受醇类型的显著影响。在最佳条件下，乙醇解后甘油酯中EPA和DHA的含量分别达到12.91%和55.40%，DHA产率为79.22%。在本研究中，一个有趣的发现是，Eversa® Transform 2.0在醇解过程中能够有效区分EPA和DHA，使我们能够在去除饱和和单不饱和乙酯后制备富含DHA的甘油酯和富含EPA的乙酯。
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