College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Food Chem. 2020 Nov 15;330:127225. doi: 10.1016/j.foodchem.2020.127225. Epub 2020 Jun 4.
The whole genome of Streptomyces violascens (=ATCC 27968) was sequenced and the cloning and expression of OUC-Lipase 6 were conducted in Bacillus subtilis WB800. The recombinant enzyme belongs to the lipolytic enzymes family V. OUC-Lipase 6 showed optimal activity at 30 °C and pH 9.0, and retained 90.2% of its activity in an alkaline buffer (pH 8.0, 30 °C and 96 h). OUC-Lipase 6 showed good stability under medium temperature conditions (residual activity of 68.8%, pH 8.0, 45 °C and 96 h). OUC-Lipase 6 could selectively hydrolyze fatty acids on the glyceride backbone, thus improving the contents of DHA and EPA in codfish oil. OUC-Lipase 6 also showed regioselectivity, resulting in a better enrichment efficiency for EPA than DHA. After hydrolyzing for 36 h via OUC-Lipase 6, the contents of EPA and DHA were improved to 3.24-fold and 1.98-fold, respectively.
链霉菌紫色变种（=ATCC 27968）的全基因组被测序，脂肪酶 6 的克隆和表达在枯草芽孢杆菌 WB800 中进行。重组酶属于脂肪酶家族 V。OUC-Lipase 6 在 30°C 和 pH9.0 下表现出最佳活性，在碱性缓冲液（pH8.0，30°C 和 96h）中保留了 90.2%的活性。OUC-Lipase 6 在中温条件下具有良好的稳定性（残余活性 68.8%，pH8.0，45°C 和 96h）。OUC-Lipase 6 可以选择性地水解甘油酯主链上的脂肪酸，从而提高鳕鱼鱼油中 DHA 和 EPA 的含量。OUC-Lipase 6 还具有区域选择性，导致 EPA 的富集效率优于 DHA。通过 OUC-Lipase 6 水解 36h 后，EPA 和 DHA 的含量分别提高到 3.24 倍和 1.98 倍。
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