College of Resources and Environment, Anhui Agricultural University, Anhui Provincial Key Laboratory of Hazardous Factors and Risk Control of Agri-food Quality Safety, Hefei 230036, China.
College of Resources and Environment, Anhui Agricultural University, Anhui Provincial Key Laboratory of Hazardous Factors and Risk Control of Agri-food Quality Safety, Hefei 230036, China.
J Hazard Mater. 2024 Dec 5;480:135773. doi: 10.1016/j.jhazmat.2024.135773. Epub 2024 Sep 6.
The extensive use of pharmaceutical and personal care products (PPCPs) has led to widespread residual pollution, which increases the risk of the development of drug resistance in pathogenic microorganisms. Benzocaine is a PPCP that is widely used medical anesthesia and in sunscreen. Microorganisms are essential for the degradation of residual PPCPs. However, no studies have reported the microbial degradation of benzocaine. In this study, through continuous enrichment of the initial consortium HJ1, the highly efficient benzocaine-degrading consortium HJ7 was obtained, HJ7 exhibited a degradation rate that was 1.92 times greater than that of HJ1. Methyl 4-aminobenzoate and 4-aminobenzoic acid were identified as major intermediate products during benzocaine biodegradation by consortium HJ1 or HJ7. Methylobacillus (57.8 % ± 0.9 %) and Pseudomonas (22.1 % ± 0.7 %), which are thought to harbor essential species for benzocaine degradation, were significantly enriched in consortium HJ7. Two benzocaine-degrading strains, Pseudomonas sp. A8 and Microbacterium sp. A741, and one methyl 4-aminobenzoate-degrading strain, Achromobacter sp. A5, were isolated from consortium HJ7, and they synergistically mineralized benzocaine. These findings not only provide new insights into the biotransformation of benzocaine but also provide strain resources for the bioremediation of residual benzocaine in the environment.
制药和个人护理产品（PPCPs）的广泛使用导致了广泛的残留污染，增加了病原微生物产生耐药性的风险。苯佐卡因是一种广泛用于医疗麻醉和防晒霜的 PPCP。微生物是残留 PPCPs 降解的关键。然而，目前还没有研究报道苯佐卡因的微生物降解。在这项研究中，通过对初始混合菌群 HJ1 的连续富集，得到了高效降解苯佐卡因的混合菌群 HJ7，HJ7 的降解速率比 HJ1 高 1.92 倍。通过混合菌群 HJ1 或 HJ7 降解苯佐卡因，鉴定出了主要的中间产物是 4-氨基苯甲酸甲酯和 4-氨基苯甲酸。甲基杆菌（57.8%±0.9%）和假单胞菌（22.1%±0.7%）在混合菌群 HJ7 中明显富集，被认为是苯佐卡因降解的关键种。从混合菌群 HJ7 中分离出了两种苯佐卡因降解菌株，即假单胞菌 A8 和微杆菌 A741，以及一种 4-氨基苯甲酸甲酯降解菌株，即不动杆菌 A5，它们协同矿化苯佐卡因。这些发现不仅为苯佐卡因的生物转化提供了新的见解，也为环境中残留苯佐卡因的生物修复提供了菌株资源。
