Department of Medical, Oral and Biotechnological Sciences, University of "G. d'Annunzio" Chieti-Pescara, 66100 Chieti, Italy.
Department of Pharmacy, University of "G. d'Annunzio" Chieti-Pescara, 66100 Chieti, Italy.
J Appl Microbiol. 2024 Sep 2;135(9). doi: 10.1093/jambio/lxae234.
Bacterial biofilms can form on surfaces in hospitals, clinics, farms, and food processing plants, representing a possible source of infections and cross-contamination. This study investigates the effectiveness of new commercial wipes against Staphylococcus aureus and Pseudomonas aeruginosa biofilms (early attachment and formed biofilms), assessing LH SALVIETTE wipes (Lombarda H S.r.l.) potential for controlling biofilm formation.
The wipes efficacy was studied against the early attachment phase and formed biofilm of S. aureus ATCC 6538 and P. aeruginosa ATCC 15442 on a polyvinyl chloride (PVC) surface, following a modified standard test EN 16615:2015, measuring Log10 reduction and cell viability using live/dead staining. It was also evaluated the wipes anti-adhesive activity over time (3 h, 2 4h), calculating CFU.mL-1 reduction. Data were analyzed using t-student test. The wipes significantly reduced both early phase and formed S. aureus biofilm, preventing dispersion on PVC surfaces. Live/dead imaging showed bacterial cluster disaggregation and killing action. The bacterial adhesive capability decreased after short-time treatment (3 h) with the wipes compared to 24 h.
Results demonstrated decreased bacterial count on PVC surface both for early attachment phase and formed biofilms, also preventing the bacterial biofilm dispersion.
细菌生物膜可以在医院、诊所、农场和食品加工厂的表面形成，这代表了一种可能的感染源和交叉污染。本研究调查了新的商业湿巾对金黄色葡萄球菌和铜绿假单胞菌生物膜（早期附着和形成的生物膜）的有效性，评估了 LH SALVIETTE 湿巾（Lombarda H S.r.l.）控制生物膜形成的潜力。
根据修改后的标准测试 EN 16615:2015，使用活/死染色法测量 Log10 减少和细胞活力，研究了湿巾对金黄色葡萄球菌 ATCC 6538 和铜绿假单胞菌 ATCC 15442 在聚氯乙烯（PVC）表面上的早期附着阶段和形成生物膜的效果。还评估了湿巾在不同时间段（3 h、24 h）的抗粘附活性，计算 CFU.mL-1 的减少量。使用 t 检验对数据进行分析。湿巾显著减少了金黄色葡萄球菌早期附着阶段和形成的生物膜，防止了其在 PVC 表面上的分散。活/死成像显示了细菌簇的解聚和杀伤作用。与 24 h 相比，湿巾在短时间（3 h）处理后，细菌的粘附能力降低。
结果表明，在 PVC 表面上，无论是早期附着阶段还是形成的生物膜，细菌数量都减少了，并且还防止了细菌生物膜的分散。
