Ismael Mohamedelfatieh, Qayyum Nageena, Gu Yaxin, Na Li, Haoyue Han, Farooq Muhammad, Wang Panpan, Zhong Qingping, Lü Xin
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Lab of Bioresource, College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China.
Probiotics Antimicrob Proteins. 2024 Sep 13. doi: 10.1007/s12602-024-10356-7.
Multidrug-resistant Escherichia coli (MDR-E. coli) is a global health concern. Lactic acid bacteria (LAB) are important probiotics that have beneficial effects on health, and in recent years, their influences in preventing foodborne pathogens-induced colitis have attracted much attention. Therefore, this study aimed to investigate the oral administration of Lactiplantibacillus plantarum NWAFU-BIO-BS29 as an emerging approach to alleviate MDR-E. coli-induced colitis in BALB/c mice model. To illustrate the mode of action of NWAFU-BIO-BS29 interventions with the gut microbiota and immune responses, the changes on the colonic mucosal barrier, regulatory of the gene expressions of inflammatory cytokines, re-modulating the intestinal microflora, and changes in physiological parameters were studied. The results indicated that daily supplementation of 200 µL fresh bacteria for 7 days had ameliorated the associated colitis and partially prevented the infection. The modes of action by ameliorating the inflammatory response, which destructed villous and then affected the intestinal barrier integrity, reducing the secretion of interleukins (6 and β) and tumor necrosis factor (TNF-α) in serum by 87.88-89.93%, 30.73-35.98%, and 19.14-22.32%, respectively, enhancing the expressions of some epithelial integrity-related proteins in the mouse mucous layer of mucins 2 and 3, Claudin-1, and Occludin by 130.00-661.85%, 27.64-57.35%, 75.52-162.51%, and 139.36-177.73%, respectively, and 56.09-73.58% for toll-like receptor (TLR4) in colon tissues. Notably, the mouse gut microbiota analysis showed an increase in the relative abundance of beneficial bacteria, including Lactobacillus, Bacteriodales bacterium, Candidatus Saccharimonas, Enterorhabdus, and Bacilli. Furthermore, the probiotic promoted the proliferation of epithelia and goblet cells by increasing short-chain fatty acids (SCFAs) levels by 19.23-31.39%. In conclusion, L. plantarum NWAFU-BIO-BS29 has potential applications and can be considered a safe dietary supplement to ameliorate the colitis inflammation symptoms of MDR-E. coli infection.
多重耐药性大肠杆菌（MDR-E. coli）是一个全球关注的健康问题。乳酸菌（LAB）是对健康有益的重要益生菌，近年来，它们在预防食源性病原体诱导的结肠炎方面的作用备受关注。因此，本研究旨在探讨口服植物乳杆菌NWAFU-BIO-BS29作为一种新方法，以减轻BALB/c小鼠模型中MDR-E. coli诱导的结肠炎。为了阐明NWAFU-BIO-BS29干预肠道微生物群和免疫反应的作用模式，研究了结肠黏膜屏障的变化、炎性细胞因子基因表达的调控、肠道微生物群的重新调节以及生理参数的变化。结果表明，连续7天每天补充200µL新鲜细菌可改善相关结肠炎并部分预防感染。其作用模式包括改善炎症反应，炎症反应破坏绒毛进而影响肠道屏障完整性，使血清中白细胞介素（6和β）和肿瘤坏死因子（TNF-α）的分泌分别减少87.88 - 89.93%、30.73 - 35.98%和19.14 - 22.32%，使小鼠黏液层中黏蛋白2和3、Claudin-1和Occludin等一些与上皮完整性相关蛋白的表达分别提高130.00 - 661.85%、27.64 - 57.35%、75.52 - 162.51%和139.36 - 177.73%，结肠组织中Toll样受体（TLR4）提高56.09 - 73.58%。值得注意的是，小鼠肠道微生物群分析显示有益细菌的相对丰度增加，包括乳酸杆菌、拟杆菌属细菌、候选糖单胞菌属、肠道杆菌属和芽孢杆菌属。此外，该益生菌通过使短链脂肪酸（SCFAs）水平提高19.23 - 31.39%，促进上皮细胞和杯状细胞的增殖。总之，植物乳杆菌NWAFU-BIO-BS29具有潜在应用价值，可被视为一种安全的膳食补充剂，用于改善MDR-E. coli感染的结肠炎炎症症状。
