Yi Gyeong-Seon, Jin Xiangji, Zheng Qiwen, Nguyen Trang Thi Minh, Yang Su-Jin, Yi Tae-Hoo
Department of Convergent Biotechnology and Advanced Materials Engineering, Graduate School, Kyung Hee University, Yongin 17104, Republic of Korea.
Department of Dermatology, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea.
Antibiotics (Basel). 2025 Apr 8;14(4):389. doi: 10.3390/antibiotics14040389.
: Multidrug-resistant (MDR) pathogens pose a critical challenge in infection treatment. () is known for its antimicrobial activity; however, studies on its effects against MDR pathogens remain limited. This study aimed to evaluate the antimicrobial and biological activities of PMY2, isolated from fermented porcine colostrum yogurt, against MDR pathogens, including (), (), and (). : The antimicrobial, anti-inflammatory, and cytotoxic effects of PMY2 were evaluated in vitro. In addition, IL-6 and TNF-α levels were analyzed using an ELISA kit. : The MIC value against KCTC 3881 and MRSA (CCARM 3089) was 0.31 mg/mL, while the MBC values were 0.63 mg/mL and 2.5 mg/mL, respectively. At MIC, biofilm formation was inhibited by 62.2% in KCTC 3881 and by 51.5% in MRSA. CFS exhibited low cytotoxicity in RAW 264.7 macrophages and significantly reduced NO production, IL-6, and TNF-α levels, indicating strong anti-inflammatory effects. : These findings suggest that PMY2 exhibited excellent antimicrobial and anti-inflammatory activity against MDR pathogens, demonstrating its potential as a natural antimicrobial agent. These results indicate that PMY2 CFS could be a promising candidate for addressing antibiotic resistance issues.
多重耐药（MDR）病原体在感染治疗中构成了严峻挑战。（某物质）以其抗菌活性而闻名；然而，关于其对多重耐药病原体影响的研究仍然有限。本研究旨在评估从发酵猪初乳酸奶中分离出的PMY2对包括（某些病原体）、（某些病原体）和（某些病原体）在内的多重耐药病原体的抗菌和生物学活性。：在体外评估了PMY2的抗菌、抗炎和细胞毒性作用。此外，使用酶联免疫吸附测定试剂盒分析了白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）水平。：对韩国典型培养物保藏中心（KCTC）3881和耐甲氧西林金黄色葡萄球菌（MRSA，韩国微生物保藏中心3089）的最低抑菌浓度（MIC）值为0.31毫克/毫升，而最低杀菌浓度（MBC）值分别为0.63毫克/毫升和2.5毫克/毫升。在MIC浓度下，KCTC 3881中的生物膜形成被抑制了62.2%，MRSA中的生物膜形成被抑制了51.5%。发酵上清液（CFS）在RAW 264.7巨噬细胞中表现出低细胞毒性，并显著降低了一氧化氮（NO）的产生、IL-6和TNF-α水平，表明其具有强大的抗炎作用。：这些发现表明，PMY2对多重耐药病原体表现出优异的抗菌和抗炎活性，证明了其作为天然抗菌剂的潜力。这些结果表明，PMY2 CFS可能是解决抗生素耐药性问题的一个有前途的候选物。
