Huang Ailan, Wu Xinming, Lu Fuping, Liu Fufeng
School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453000, China.
College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
Foods. 2024 Sep 5;13(17):2820. doi: 10.3390/foods13172820.
Ulvan is a water-soluble sulfated polysaccharide extracted from the green algae cell wall. Compared with polysaccharides, oligosaccharides have drawn increasing attention in various industries due to their enhanced biocompatibility and solubility. Ulvan lyase degrades polysaccharides into low molecular weight oligosaccharides through the -elimination mechanism. The elucidation of the structure, catalytic mechanism, and molecular modification of ulvan lyase will be helpful to obtain high value-added products from marine biomass resources, as well as reduce environmental pollution caused by the eutrophication of green algae. This review summarizes the structure and bioactivity of ulvan, the microbial origin of ulvan lyase, as well as its sequence, three-dimensional structure, and enzymatic mechanism. In addition, the molecular modification of ulvan lyase, prospects and challenges in the application of enzymatic methods to prepare oligosaccharides are also discussed. It provides information for the preparation of bioactive oligosaccharides through enzymatic hydrolysis, the technological bottlenecks, and possible solutions to address these issues within the enzymatic process.
岩藻聚糖硫酸酯是一种从绿藻细胞壁中提取的水溶性硫酸化多糖。与多糖相比，低聚糖因其增强的生物相容性和溶解性而在各个行业中受到越来越多的关注。岩藻聚糖硫酸酯酶通过β-消除机制将多糖降解为低分子量的低聚糖。阐明岩藻聚糖硫酸酯酶的结构、催化机制和分子修饰，将有助于从海洋生物质资源中获得高附加值产品，并减少绿藻富营养化造成的环境污染。本文综述了岩藻聚糖硫酸酯的结构和生物活性、岩藻聚糖硫酸酯酶的微生物来源及其序列、三维结构和酶促机制。此外，还讨论了岩藻聚糖硫酸酯酶的分子修饰、酶法制备低聚糖应用中的前景和挑战。它为通过酶水解制备生物活性低聚糖、技术瓶颈以及在酶促过程中解决这些问题的可能方案提供了信息。
