Lahaye M, Brunel M, Bonnin E
Institut National de la Recherche Agronomique, Laboratoire de Biochimie et Technologie des Glucides, Nantes, France.
Carbohydr Res. 1997 Nov 28;304(3-4):325-33. doi: 10.1016/s0008-6215(97)00270-x.
A marine bacterium degrading the water-soluble cell wall polysaccharides from Ulva sp. (ulvan) has been isolated. The good correlation between ulvan degradation monitored by reducing-power, UV absorbance and viscosimetry, indicated that the crude enzymatic extract contains essentially an endo-ulvan lyase activity. This activity was rapidly inhibited by the reaction products which consisted of a series of ulvanobiouronic acid A 3-sulfate [-->4)-beta-D-GlcpA-(1-->4)-alpha-L-Rhap 3-sulfate-(1-->]n with 4-deoxy-L-threo-hex-4-enopyranosiduronic acid at the non-reducing end. Other deviant repeating structures with beta-D-Xylp or alpha-IdopA replacing beta-D-GlcpA in the repeating ulvanobiouronic acid disaccharide and the presence of two consecutive (1-->4) linked beta-D-Glc pA demonstrated the great variability and complexity of ulvan chemical structure.
一株能降解石莼属（石莼聚糖）水溶性细胞壁多糖的海洋细菌已被分离出来。通过还原力、紫外吸光度和粘度测定法监测石莼聚糖降解之间的良好相关性表明，粗酶提取物基本上含有一种内切石莼聚糖裂解酶活性。该活性被由一系列3 - 硫酸化的石莼二糖醛酸A [-->4)-β-D-葡萄糖醛酸-(1-->4)-α-L-鼠李糖3 - 硫酸酯-(1-->]n组成的反应产物迅速抑制，其在非还原端带有4 - 脱氧-L-苏式-己-4-烯吡喃糖醛酸。在重复的石莼二糖醛酸二糖中，其他以β-D-木糖或α-L-艾杜糖醛酸取代β-D-葡萄糖醛酸的异常重复结构以及两个连续的(1-->4)连接的β-D-葡萄糖醛酸的存在，证明了石莼聚糖化学结构的巨大变异性和复杂性。
