Roth C, Rocca-Serra J, Sommé G, Fougereau M, Théze J
Proc Natl Acad Sci U S A. 1985 Jul;82(14):4788-92. doi: 10.1073/pnas.82.14.4788.
Eight monoclonal antibodies were selected from BALB/c mice immunized with two different monoclonal anti-idiotypic antibodies recognizing two discrete idiotopes characteristic of the anti-poly(Glu60Ala30Tyr10) (GAT) antibody response. These monoclonal antibodies were previously classified as Ab1 (anti-GAT-like) and Ab3 (anti-anti-idiotype) on the basis of expression of the public idiotypic specificity (p.GAT) studied with a xenogeneic serum, anti-GAT activity, and expression of various public idiotopes. All the heavy chain variable region (VH) sequences from Ab1 are nearly identical to the VH sequences of Ab1 anti-GAT monoclonal antibodies. The same type of results has been found with the Ab1 kappa light chain variable region (V kappa) sequences. Confirming our classification, Ab3 VH and V kappa sequences were found to be completely different from Ab1 VH and V kappa sequences. The Ab1 diversity (D) regions are different from one another and different from the D regions found on monoclonal anti-GAT antibodies but function similarly. These D regions are not simply derived from already described D genes. Finally, our results suggest that in the anti-GAT response VH and V kappa sequence are mainly responsible for idiotype expression.
从用两种不同的单克隆抗独特型抗体免疫的BALB/c小鼠中筛选出8种单克隆抗体，这两种单克隆抗独特型抗体识别抗聚（Glu60Ala30Tyr10）（GAT）抗体应答所特有的两个离散独特位。这些单克隆抗体先前根据用异种血清研究的公共独特型特异性（p.GAT）的表达、抗GAT活性以及各种公共独特位的表达被分类为Ab1（抗GAT样）和Ab3（抗抗独特型）。来自Ab1的所有重链可变区（VH）序列与Ab1抗GAT单克隆抗体的VH序列几乎相同。在Ab1κ轻链可变区（Vκ）序列中也发现了相同类型的结果。证实我们的分类，发现Ab3的VH和Vκ序列与Ab1的VH和Vκ序列完全不同。Ab1的多样性（D）区彼此不同，也与单克隆抗GAT抗体上发现的D区不同，但功能相似。这些D区并非简单地源自已描述的D基因。最后，我们的结果表明，在抗GAT应答中，VH和Vκ序列主要负责独特型的表达。
