Baird A, Mormède P, Ying S Y, Wehrenberg W B, Ueno N, Ling N, Guillemin R
Proc Natl Acad Sci U S A. 1985 Aug;82(16):5545-9. doi: 10.1073/pnas.82.16.5545.
The addition of fibroblast growth factor (FGF) to primary cultures of rat anterior pituitary cells modifies their response to thyrotropin-releasing factor in a dose-dependent manner. While the pituitary response to the other releasing factors (corticotropin-releasing factor, growth hormone-releasing factor, and gonadotropin-releasing factor) is not altered, FGF increases both the sensitivity of the cells to thyrotropin-releasing factor and the amounts of prolactin and thyrotropin released. A minimum of 24 hr of preincubation with FGF is required to modify the pituitary response, and maximal effects were observed with 48 and 72 hr of preincubation. The effective doses of FGF are similar to those described for its mitogenic activity (i.e., 1-100 pM), but inhibition of cell growth with 5-fluorodeoxyuridine does not modify the effect of FGF on thyrotropin and prolactin release. These results suggest a novel paracrine, if not autocrine, role of pituitary FGF in the homeostatic mechanisms that regulate the secretion of prolactin and thyrotropin. They also suggest that the biological significance of the presence of FGF in various tissues may not be directly related to its in vitro mitogenic activity.
向大鼠垂体前叶细胞原代培养物中添加成纤维细胞生长因子（FGF）会以剂量依赖的方式改变它们对促甲状腺激素释放因子的反应。虽然垂体对其他释放因子（促肾上腺皮质激素释放因子、生长激素释放因子和促性腺激素释放因子）的反应没有改变，但FGF会增加细胞对促甲状腺激素释放因子的敏感性以及催乳素和促甲状腺激素的释放量。至少需要用FGF预孵育24小时才能改变垂体反应，预孵育48小时和72小时时观察到最大效应。FGF的有效剂量与其促有丝分裂活性所描述的剂量相似（即1-100 pM），但用5-氟脱氧尿苷抑制细胞生长并不会改变FGF对促甲状腺激素和催乳素释放的影响。这些结果表明垂体FGF在调节催乳素和促甲状腺激素分泌的稳态机制中具有一种新的旁分泌（甚至可能是自分泌）作用。它们还表明，FGF在各种组织中的生物学意义可能与其体外促有丝分裂活性没有直接关系。
