College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China.
Beijing Laboratory of Urban and Rural Ecological Environment, Beijing 102206, China.
Int J Mol Sci. 2024 Aug 23;25(17):9156. doi: 10.3390/ijms25179156.
is one of the most widely cultivated ornamental bulbous plants in the world. Although research has shown that variable temperature treatments can accelerate the development process from vegetative to reproductive growth in , the molecular regulation mechanisms of this development are not clear. In this study, -miR171b and its target gene, , were selected and validated using transgenic functional verification, subcellular localization, and transcriptional activation. This study also investigated the differential expression of -miR171b and in two temperature treatment groups (25 °C and 15 °C). -miR171b expression significantly increased after the temperature change, whereas that of exhibited the opposite trend. Through in situ hybridization experiments facilitated by the design of hybridization probes targeting , a reduction in expression was detected following variable temperature treatment at 15 °C. The transgenic overexpression of -miR171b in plants promoted the phase transition, while overexpression induced a delay in the phase transition. In addition, LbrWOX4 interacted with LbrSCL6 in yeast two-hybrid and bimolecular fluorescence complementation assays. In conclusion, these results explain the molecular regulatory mechanisms governing the phase transition in .
是世界上栽培最广泛的观赏鳞茎植物之一。虽然研究表明，变温处理可以加速从营养生长到生殖生长的发育过程，但这一发育的分子调控机制尚不清楚。在这项研究中，通过转基因功能验证、亚细胞定位和转录激活，选择并验证了-miR171b 及其靶基因 。本研究还调查了 -miR171b 和 在两个温度处理组（25°C 和 15°C）中的差异表达。-miR171b 的表达在温度变化后显著增加，而 的表达则呈现相反的趋势。通过针对 的杂交探针设计进行的原位杂交实验，在 15°C 的变温处理后检测到 的表达减少。植物中 -miR171b 的过表达促进了阶段转变，而过表达则诱导阶段转变延迟。此外，LbrWOX4 在酵母双杂交和双分子荧光互补测定中与 LbrSCL6 相互作用。总之，这些结果解释了控制 阶段转变的分子调控机制。
