College of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, People's Republic of China.
Beijing Laboratory of Urban and Rural Ecological Environment, Beijing, 102206, People's Republic of China.
Planta. 2023 Jul 29;258(3):53. doi: 10.1007/s00425-023-04208-x.
Lbr-miR172a could promote the growth phase transition and shorten maturation in Lilium, while LbrTOE3 inhibited this process and prolonged the growth period. Lilium is an ornamental flower with high economic value for both food and medicinal purposes. However, under natural conditions, Lilium bulbs take a long time and cost more to grow to commercial size. This research was conducted to shorten the maturation time by subjecting Lilium bulbs to alternating temperature treatment. To explore the molecular mechanism of the vegetative phase change (VPC) in Lilium after variable temperature treatment, the key module miR172a-TOE3 was selected based on a combined omics analysis. Gene cloning and transgene functional validation showed that overexpression of Lbr-mir172a promoted a phase change, while overexpression of LbrTOE3 inhibited this process. Subcellular localization and transcriptional activation assays indicated that LbrTOE3 was predominantly localized in the nucleus and showed transcriptional activity. In situ hybridization showed that LbrTOE3 expression was significantly downregulated after alternating temperature treatment. This study elucidates the molecular mechanisms of the phase transition of Lilium and provides a scientific basis for the phase transition in other plants.
Lbr-miR172a 可促进百合生长阶段的转变并缩短其成熟期，而 LbrTOE3 则抑制这一过程并延长生长周期。百合是一种具有高经济价值的观赏花卉，可用于食品和药用。然而，在自然条件下，百合鳞茎需要很长时间和更高的成本才能生长到商业规模。本研究旨在通过对百合鳞茎进行变温处理来缩短其成熟期。为了探索百合在变温处理后营养生长向生殖生长转变的分子机制，我们基于联合组学分析，选择了关键的 miR172a-TOE3 模块。基因克隆和转基因功能验证表明，Lbr-mir172a 的过表达促进了转变，而 LbrTOE3 的过表达则抑制了这一过程。亚细胞定位和转录激活实验表明，LbrTOE3 主要定位于细胞核并具有转录活性。原位杂交显示，LbrTOE3 的表达在变温处理后显著下调。本研究阐明了百合转变的分子机制，为其他植物的转变提供了科学依据。
