College of Animal & Verterinary Sciences, Southwest Minzu University, Chengdu 610041, China.
Key Laboratory of Qinghai-Tibet Plateau Animal Genetic Resource and Utilization, Ministry of Education, Southwest Minzu University, Chengdu 610041, China.
Int J Mol Sci. 2024 Aug 30;25(17):9431. doi: 10.3390/ijms25179431.
Yak is an excellent germplasm resource on the Tibetan Plateau and is able to live in high-altitude areas with hypoxic, cold, and harsh environments. Studies on induced pluripotent stem cells (iPSCs) in large ruminants commonly involve a combination strategy involving six transcription factors, , , , , , and (OSKMNL). This strategy tends to utilize genes from the same species to optimize pluripotency maintenance. In this study, we cloned the six pluripotency genes (OSKMNL) from yak and constructed a multi-cistronic lentiviral vector carrying these genes. This vector efficiently delivered the genes into yak fibroblasts, aiming to promote the reprogramming process. We verified that the treated cells had several pluripotency characteristics, marking the first successful construction of a lentiviral system carrying yak pluripotency genes. This achievement lays the foundation for subsequent establishment of yak iPSCs and holds significant implications for yak-breed improvement and germplasm-resource conservation.
牦牛是青藏高原上的优秀种质资源，能够生活在缺氧、寒冷和恶劣环境的高海拔地区。大牲畜诱导多能干细胞（iPSCs）的研究通常涉及一种组合策略，涉及六个转录因子， ， ， ， ，和 （OSKMNL）。该策略倾向于利用来自同一物种的基因来优化多能性维持。在这项研究中，我们从牦牛中克隆了六个多能性基因（OSKMNL），并构建了一个携带这些基因的多顺反子慢病毒载体。该载体有效地将基因导入牦牛成纤维细胞，旨在促进重编程过程。我们验证了处理后的细胞具有几种多能性特征，标志着携带牦牛多能性基因的慢病毒系统的首次成功构建。这一成就为随后建立牦牛 iPSCs 奠定了基础，对牦牛品种改良和种质资源保护具有重要意义。
