College of Life Sciences, Jilin Agricultural University, Changchun 130118, China.
Joint International Research Laboratory of Modern Agricultural Technology, Ministry of Education, Changchun 130118, China.
Int J Mol Sci. 2024 Sep 5;25(17):9607. doi: 10.3390/ijms25179607.
Auxin response factors(ARFs) are a class of transcription factors that regulate the expression of auxin response genes and play a crucial role in plant growth and development. Florigen plays a crucial role in the process of flowering. However, the process by which auxin regulates the accumulation of florigen remains largely unclear. This study found that the expression of in maize increases during flowering, and the genetic transformation of accelerates the flowering process in and . Furthermore, was found to be positively correlated with the transcription of the gene. Similarly, the FT-like gene in rescues the late flowering phenotype of the FT mutation in Arabidopsis. Moreover, actively participates in the accumulation of florigen and the flowering process. Further research revealed that positively responds to the auxin signal, and that the interaction between ZmARF16 and the promoter, as well as the subsequent promotion of gene expression, leads to early flowering. This was confirmed through a yeast one-hybrid and dual-luciferase assay. Therefore, the study provides evidence that the - module plays a crucial role in regulating the flowering process of .
生长素响应因子(ARFs)是一类转录因子,它们调节生长素响应基因的表达,在植物生长和发育中起着关键作用。成花素在开花过程中起着关键作用。然而,生长素调节成花素积累的过程在很大程度上还不清楚。本研究发现,玉米中 的表达在开花期间增加,而 的遗传转化加速了 和 中的开花过程。此外,发现 与 基因的转录呈正相关。同样,拟南芥中 FT 突变体的晚花表型被 FT 样基因 挽救。此外, 积极参与成花素的积累和开花过程。进一步的研究表明, 对生长素信号有积极的响应,ZmARF16 与 启动子的相互作用,以及随后促进 基因的表达,导致早期开花。这通过酵母单杂交和双荧光素酶测定得到了证实。因此,该研究提供了证据表明 - 模块在调节玉米的开花过程中起着关键作用。