Fukushima Yoko, Takahashi Shizuka, Nakamura Machiko, Inoue Tatsuya, Fujieda Yusuke, Sato Toshiyuki, Noguchi Shingo, Tsujikawa Motokazu, Sakaguchi Hirokazu, Nishida Kohji
Department of Ophthalmology, Osaka University Graduate School of Medicine, Suita 565-0871, Osaka, Japan.
Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives (OTRI), Suita 565-0871, Osaka, Japan.
J Clin Med. 2024 Aug 27;13(17):5073. doi: 10.3390/jcm13175073.
: The aim of this paper was to investigate the protein concentrations of high-temperature requirement A 1 (HTRA1) and transforming growth factor-β (TGF-β) in the vitreous humor of patients with chorioretinal vascular diseases. : This study measured protein concentrations of HTRA1, TGF-β1-3, and vascular endothelial growth factor A (hereinafter called VEGF) in the vitreous humor from seven eyes of patients with chorioretinal vascular diseases (age-related macular degeneration, diabetic macular edema, and retinal vein occlusion) and six control eyes (idiopathic epiretinal membrane and macular hole). We analyzed the mutual relationship among the protein levels. : The protein levels of HTRA1 and VEGF were significantly increased in the chorioretinal vascular disease group compared with the control group (1.57 ± 0.79 ×10-9 mol/mL vs. 0.68 ± 0.79 ×10-9 mol/mL, = 0.039; 3447.00 ± 3423.47 pg/mL vs. 35.33 ± 79.01 pg/mL, = 0.046, respectively). TGF-β2 levels were not significantly different between groups (2222.71 ± 1151.25 pg/mL for the chorioretinal vascular disease group vs. 1918.83 ± 744.01 pg/mL for the control group, = 0.62). The concentration of HTRA1 was strongly associated with TGF-β2 levels in the vitreous humor, independent of VEGF (r = 0.80, = 0.0010). : We revealed that vitreous HTRA1 was increased in patients with chorioretinal vascular diseases and strongly correlated with TGF-β2.
本文旨在研究高温需求A1（HTRA1）和转化生长因子-β（TGF-β）在脉络膜视网膜血管疾病患者玻璃体液中的蛋白浓度。本研究检测了脉络膜视网膜血管疾病（年龄相关性黄斑变性、糖尿病性黄斑水肿和视网膜静脉阻塞）患者7只眼以及6只对照眼（特发性视网膜前膜和黄斑裂孔）玻璃体液中HTRA1、TGF-β1 - 3和血管内皮生长因子A（以下简称VEGF）的蛋白浓度。我们分析了这些蛋白水平之间的相互关系。与对照组相比，脉络膜视网膜血管疾病组中HTRA1和VEGF的蛋白水平显著升高（分别为1.57±0.79×10 - 9 mol/mL对0.68±0.79×10 - 9 mol/mL，P = 0.039；3447.00±3423.47 pg/mL对35.33±79.01 pg/mL，P = 0.046）。两组间TGF-β2水平无显著差异（脉络膜视网膜血管疾病组为2222.71±1151.25 pg/mL，对照组为1918.83±744.01 pg/mL，P = 0.62）。玻璃体液中HTRA1的浓度与TGF-β2水平密切相关，独立于VEGF（r = 0.80，P = 0.0010）。我们发现脉络膜视网膜血管疾病患者玻璃体液中的HTRA1升高，且与TGF-β2密切相关。
