College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Key Laboratory for Advanced Materials, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
Molecules. 2024 Aug 24;29(17):4001. doi: 10.3390/molecules29174001.
Stimulus-responsive materials hold significant promise for antitumor applications due to their variable structures and physical properties. In this paper, a series of peptides with a responsive viologen derivative, Pep-CV ( = 1, 2, 3) were designed and synthesized. The process and mechanism of the interaction were studied and discussed. An ultraviolet-visible (UV) spectrophotometer and fluorescence spectrophotometer were used to study their redox responsiveness. Additionally, their secondary structures were measured by Circular Dichroism (CD) in the presence or absence of the reductant, NaSO. DPPC and DPPG liposomes were prepared to mimic normal and tumor cell membranes. The interaction between Pep-CV and biomembranes was investigated by the measurements of surface tension and cargo leakage. Results proved Pep-CV was more likely to interact with the DPPG liposome and destroy its biomembrane under the stimulus of the reductant. And the destruction increased with the length of the hydrophobic tail chain. Pep-CV showed its potential as an intelligent antitumor agent.
刺激响应型材料由于其可变的结构和物理性质，在抗肿瘤应用中具有重要的应用前景。在本文中，设计并合成了一系列带有响应性二茂铁衍生物的肽，Pep-CV(=1,2,3)。研究并讨论了它们相互作用的过程和机制。使用紫外-可见分光光度计和荧光分光光度计研究了它们的氧化还原响应性。此外，通过在还原剂 NaSO 存在或不存在的情况下使用圆二色性（CD）测量它们的二级结构。制备 DPPC 和 DPPG 脂质体来模拟正常和肿瘤细胞膜。通过测量表面张力和货物泄漏来研究 Pep-CV 与生物膜之间的相互作用。结果证明，在还原剂的刺激下，Pep-CV 更有可能与 DPPG 脂质体相互作用并破坏其生物膜。并且，破坏程度随疏水尾链长度的增加而增加。Pep-CV 表现出作为智能抗肿瘤剂的潜力。
