Chinese Materia Medica College, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
College of Agronomy and Agricultural Engineering, Liaocheng University, Liaocheng 252000, China.
Molecules. 2024 Aug 28;29(17):4074. doi: 10.3390/molecules29174074.
Previous studies have shown that (AS) has a beneficial preventive and therapeutic effect on colitis. The fermentation of lactic acid bacteria (LAB) can alter the efficacy of AS by modifying or producing new compounds with potential bioactive properties. However, the specific components and mechanisms that enhance the efficacy are still unclear. In the present experiment, untargeted metabolomics was used to analyze the changes in active components before and after LAB fermentation of AS. The aim was to explain the mechanism of AS fermentation in treating colitis using a colitis model in mice. The results indicated that the fermentation of LAB could enhance the levels of total flavonoids and total polyphenols in FAS. Additionally, the beneficial components such as Delphinidin chloride, Diosmetin, Psoralidin, and Catechol significantly increased ( < 0.05). The colitis treatment experiment demonstrated that fermented AS could alleviate symptoms and improve the morphology of colitis in mice by enhancing antioxidant enzymes like CAT, T-SOD, and T-AOC. It also regulated the composition and abundance of intestinal flora species, such as and . The effectiveness of fermented AS was significantly superior to that of unfermented AS ( < 0.05). In conclusion, this study contributes to the application of lactic acid bacteria in AS fermentation and reveals the mechanism of fermentation AS for colitis.
先前的研究表明，当归（AS）对结肠炎具有有益的预防和治疗作用。乳酸菌（LAB）的发酵可以通过修饰或产生具有潜在生物活性的新化合物来改变 AS 的功效。然而，增强功效的具体成分和机制仍不清楚。在本实验中，我们采用非靶向代谢组学分析 LAB 发酵 AS 前后活性成分的变化。目的是利用结肠炎小鼠模型，解释 AS 发酵治疗结肠炎的机制。结果表明，LAB 发酵可以提高 FAS 中总黄酮和总多酚的水平。此外，有益成分如氯化飞燕草素、香叶木素、补骨脂素和儿茶素显著增加（<0.05）。结肠炎治疗实验表明，发酵 AS 通过增强 CAT、T-SOD 和 T-AOC 等抗氧化酶，可缓解结肠炎小鼠的症状并改善其结肠形态。它还调节肠道菌群物种的组成和丰度，如 和 。发酵 AS 的效果明显优于未发酵 AS（<0.05）。总之，本研究有助于将乳酸菌应用于 AS 发酵，并揭示了发酵 AS 治疗结肠炎的机制。
