Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Department of Burn, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
J Photochem Photobiol B. 2024 Nov;260:113024. doi: 10.1016/j.jphotobiol.2024.113024. Epub 2024 Aug 30.
Ovarian aging is a serious clinical concern. Few safe and effective methods are currently available to improve ovarian functions. Photobiomodulation (PBM) is a safe and noninvasive physical therapy that can modulate a series of biological processes. Recently, several studies have noted its potential to improve the function of ovary and reproductive cells. However, the effects of PBM treatment on natural ovarian aging remain unclear. In this study, we used a naturally reproductive aging mouse model to observe the effect of PBM on ovarian function. Young and aged female ICR mice were treated with or without PBM for 2 months. PBM was performed using a semiconductor InGaAlP laser emitting at 650 nm (80 mW, 6.7 mW/cm for 5 or 10 min, resulting in a dose of 2 or 4 J/cm, respectively). After treatment, the effects of PBM and its role in oxidative stress, inflammation, and mitochondrial function were investigated. We found that PBM (4 J/cm) effectively recovered the levels of sex hormones, increased the number of primordial and growing follicles, improved angiogenesis, and decreased cell apoptosis in naturally aged mice. Moreover, PBM reduced oxidative stress, inhibited chronic ovarian inflammation, and improved mitochondrial function in aged ovaries. Similar protective effects of PBM were observed in a hydrogen peroxide-induced oxidative stress model of human granulosa cell line (KGN) in vitro. Increased cell viability, cell proliferation, hormone secretion, mitochondrial membrane potential, and adenosine triphosphate levels and decreased apoptosis and oxidative stress were detected in KGN cells after PBM treatment. Collectively, this study suggest that PBM treatment is beneficial for restoring ovarian function in naturally reproductive aging mice and has a significant protective effect against oxidative stress damage in KGN cells. The mechanisms underlying the benefits of PBM in ovarian aging include antioxidant stress, reduction of inflammation, and preservation of mitochondrial function. Therefore, this study emphasizes the potential of PBM as a therapeutic intervention to ameliorate ovarian aging.
卵巢衰老仍是一个严重的临床问题。目前，可用的改善卵巢功能的安全且有效的方法很少。光生物调节（PBM）是一种安全且非侵入性的物理疗法，可以调节一系列的生物过程。最近，几项研究注意到其改善卵巢和生殖细胞功能的潜力。然而，PBM 治疗对自然卵巢衰老的影响尚不清楚。在这项研究中，我们使用自然生殖衰老的小鼠模型来观察 PBM 对卵巢功能的影响。年轻和衰老的 ICR 雌性小鼠接受或不接受 PBM 治疗 2 个月。使用发射波长为 650nm 的半导体 InGaAlP 激光进行 PBM（80mW，6.7mW/cm2，5 或 10min，剂量分别为 2 或 4J/cm2）。治疗后，研究了 PBM 的作用及其在氧化应激、炎症和线粒体功能中的作用。我们发现，PBM（4J/cm2）有效恢复了性激素水平，增加了原始卵泡和生长卵泡的数量，改善了血管生成，并减少了自然衰老小鼠的细胞凋亡。此外，PBM 降低了氧化应激，抑制了慢性卵巢炎症，并改善了衰老卵巢中的线粒体功能。在体外过氧化氢诱导的人颗粒细胞系（KGN）氧化应激模型中也观察到了 PBM 的类似保护作用。PBM 处理后，KGN 细胞的细胞活力、细胞增殖、激素分泌、线粒体膜电位和三磷酸腺苷水平增加，凋亡和氧化应激减少。总之，这项研究表明，PBM 治疗有益于恢复自然生殖衰老小鼠的卵巢功能，并对 KGN 细胞的氧化应激损伤具有显著的保护作用。PBM 在卵巢衰老中的益处的机制包括抗氧化应激、减少炎症和保护线粒体功能。因此，这项研究强调了 PBM 作为一种治疗干预措施改善卵巢衰老的潜力。
