Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao, Shandong 266100, China; State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao, Shandong 266237, China.
Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao, Shandong 266100, China; College of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China.
J Hazard Mater. 2024 Dec 5;480:135783. doi: 10.1016/j.jhazmat.2024.135783. Epub 2024 Sep 12.
Ofloxacin (OFL), one of the most widely used fluoroquinolone antibiotics, has been frequently detected in marine environments. Nonetheless, researchers are yet to focus on the effects of OFL on the benthos. In the present study, marine clams (Ruditapes philippinarum) were exposed to OFL (0.5, 50, and 500 μg/L) for 14 d, followed by a 7 d depuration period. The accumulation of OFL, antioxidative defense responses, neurotoxicity, burrowing behavior, and metabolomic changes in clams were evaluated. The results indicated that OFL could accumulate in clams, albeit with a low bioaccumulation capacity. The intermediate (50 μg/L) and high (500 μg/L) levels of OFL induced significant antioxidative responses in the gills and digestive glands of clams, mainly manifesting as the inhibition of catalase activities and the induction of superoxide dismutase and glutathione S-transferase activities, which ultimately elevated the content of malondialdehyde, causing oxidative damage. Furthermore, the significant induction of acetylcholinesterase activities was observed, coinciding with a significant increase in burrowing rates of clams. The high level of OFL affected glycerophospholipid, arachidonic acid, steroid hormone biosynthesis, unsaturated fatty acids biosynthesis, and glycolysis/glycogenesis metabolism. In conclusion, this study has contributed to the understanding of the physiological and biochemical effects and molecular toxicity mechanisms of OFL to marine bivalves.
氧氟沙星（OFL）是最广泛使用的氟喹诺酮类抗生素之一，已在海洋环境中频繁检出。然而，研究人员尚未关注 OFL 对底栖生物的影响。在本研究中，采用海洋蛤仔（Ruditapes philippinarum）进行了 14 d 的 OFL（0.5、50 和 500μg/L）暴露实验，随后进行了 7 d 的净化期。评估了 OFL 在蛤仔体内的积累、抗氧化防御反应、神经毒性、穴居行为和代谢组学变化。结果表明，OFL 可以在蛤仔体内积累，尽管生物积累能力较低。中间（50μg/L）和高（500μg/L）浓度的 OFL 诱导了蛤仔鳃和消化腺中显著的抗氧化反应，主要表现为过氧化氢酶活性的抑制和超氧化物歧化酶和谷胱甘肽 S-转移酶活性的诱导，最终导致丙二醛含量升高，造成氧化损伤。此外，还观察到乙酰胆碱酯酶活性的显著诱导，同时蛤仔的穴居率显著增加。高水平的 OFL 影响甘油磷脂、花生四烯酸、甾体激素生物合成、不饱和脂肪酸生物合成和糖酵解/糖异生代谢。总之，本研究有助于了解 OFL 对海洋双壳类动物的生理生化影响和分子毒性机制。
