Institute of Animal Husbandry, Gödöllő Campus, Hungarian University of Agriculture and Life Sciences, H-2100 Gödöllő, Hungary.
Institute of Animal Husbandry, Gödöllő Campus, Hungarian University of Agriculture and Life Sciences, H-2100 Gödöllő, Hungary.
Aquat Toxicol. 2024 Nov;276:107076. doi: 10.1016/j.aquatox.2024.107076. Epub 2024 Sep 2.
The present study aimed to investigate the short-time (24 h) effect of aflatoxin B1 (AFB1) and sterigmatocystin (STC) on the expression of hsp70, p53, gadd45, and ogg1 genes in common carp hepatopancreas. Our results showed that aflatoxin B1 and sterigmatocystin can stimulate the expression of DNA repair genes, mainly by hour 24. This significant finding contributes to our understanding of the short-term effects of these mycotoxins on ogg1 genes in common carp hepatopancreas. One-year-old common carp juveniles were randomly distributed into five groups (Control, AFB1 0.4 mg kg feed, STC1 1 mg kg feed, STC2 2 mg kg feed, and STC3 3 mg kg feed). Hepatopancreas samples were collected three times (8, 16, and 24 h) in each group. No significant ogg1 and p53 expression changes were observed at 8 and 16 h after exposure. All measured genes were upregulated by the 24th hour in aflatoxin and STC3 groups. An increase in hsp70 gene expression was detected in all groups and all sampling. A significant decrease in gadd45aa gene expression was observed in the aflatoxin B1 group at hour 8. At hour 16, there was no significant change, while at hour 24, all treated groups were significantly different from the control. In summary, our results suggest that aflatoxin B1 and sterigmatocystin can stimulate the expression of DNA repair genes, mainly by hour 24. Further investigations are needed to get information about DNA damage parallel to the DNA repair mechanisms.
本研究旨在探究黄曲霉毒素 B1（AFB1）和杂色曲霉素（STC）在短时间（24 小时）内对鲤鱼肝胰腺中 hsp70、p53、gadd45 和 ogg1 基因表达的影响。结果表明，AFB1 和 STC 可刺激 DNA 修复基因的表达，主要在 24 小时时达到高峰。这一重要发现有助于我们了解这些霉菌毒素对鲤鱼肝胰腺 ogg1 基因的短期影响。将 1 龄鲤鱼幼鱼随机分为五组（对照组、AFB1 0.4 mg kg 饲料组、STC1 1 mg kg 饲料组、STC2 2 mg kg 饲料组和 STC3 3 mg kg 饲料组）。在每组中分别于 3 个时间点（8、16 和 24 小时）采集肝胰腺样本。暴露后 8 和 16 小时，未观察到 ogg1 和 p53 表达发生明显变化。在 AFB1 和 STC3 组，所有测定的基因在 24 小时时均被上调。所有组和所有采样时间均检测到 hsp70 基因表达增加。在 AFB1 组，gadd45aa 基因表达在 8 小时时显著下降。在 16 小时时，无明显变化，而在 24 小时时，所有处理组与对照组均有显著差异。综上所述，本研究结果表明，AFB1 和杂色曲霉素可刺激 DNA 修复基因的表达，主要在 24 小时时达到高峰。需要进一步研究以获得与 DNA 修复机制平行的 DNA 损伤信息。
