Department of Nutrition, Szent István University, H-2100 Gödöllő, Hungary.
Mycotoxins in the Food Chain Research Group, Hungarian Academy of Sciences, Kaposvár Campus, Szent István University, H-7400 Kaposvár, Hungary.
Toxins (Basel). 2021 Feb 2;13(2):109. doi: 10.3390/toxins13020109.
The purpose of the study was to evaluate the short-term effects of aflatoxin B1 (AFB1 100 µg/kg feed) and sterigmatocystin (STC 1000 μg/kg feed) exposure individually and in combination (100 μg AFB1 + 1000 μg STC/kg feed) on the parameters of lipid peroxidation and glutathione redox system both in biochemical and gene expression levels in one-year-old common carp. Lipid peroxidation parameters were slightly affected, as significant differences were observed only in conjugated diene and triene concentrations. Reduced glutathione content decreased more markedly by STC than AFB1 or AFB1+STC, but glutathione peroxidase activity did not change. Expression of and genes was down-regulated due to STC compared to AFB1 or AFB1+STC, while an induction was found as effect of AFB1+STC in the case of but down-regulation for as compared to AFB1. Expression of the glutathione biosynthesis regulatory gene, , was higher, but glutathione recycling enzyme encoding gene, was lower as an effect of AFB1+STC compared to AFB1. These results are supported by the changes in the expression of transcription factors encoding genes, and The results revealed that individual effects of AFB1 and STC on different parameters are synergistic or antagonistic in multi-toxin treatment.
本研究旨在评估黄曲霉毒素 B1（AFB1，饲料中 100µg/kg）和桔青霉素（STC，饲料中 1000µg/kg）单独及联合（100µg AFB1+1000µg STC/kg 饲料）暴露对 1 龄鲤鱼生化和基因表达水平脂质过氧化和谷胱甘肽氧化还原系统参数的短期影响。脂质过氧化参数仅在共轭二烯和三烯浓度方面存在显著差异，受到轻微影响。与 AFB1 或 AFB1+STC 相比，STC 使还原型谷胱甘肽含量下降更为明显，但谷胱甘肽过氧化物酶活性未发生变化。与 AFB1 或 AFB1+STC 相比，STC 导致 和 基因的表达下调，而 AFB1+STC 对 基因的表达诱导，对 基因的表达下调。谷胱甘肽生物合成调节基因 的表达上调，而谷胱甘肽循环酶编码基因 的表达下调，这与 AFB1+STC 相比 AFB1 的表达情况一致。转录因子编码基因 和 的表达变化支持了这些结果。这些结果表明，AFB1 和 STC 对不同参数的单独作用在多毒素处理中具有协同或拮抗作用。
