Tanaka T, Ito S, Hiroshima O, Hayashi H, Hayaishi O
Biochim Biophys Acta. 1985 Aug 22;836(1):125-33.
Six different monoclonal antibodies directed against prostaglandin E2 were obtained from hybrid myelomas, following fusion of mouse NS-1 myeloma cells with spleen cells from a rat immunized with bovine serum albumin conjugates of prostaglandin E2. Four of them were of the IgG2a subclass and the other two were an IgG2b and an IgG2c. Affinities of antibodies for prostaglandin E2 were in the range 5.8 X 10(6)-6.7 X 10(8) M-1. Cross-reactivity experiments showed that one monoclonal antibody was directed almost exclusively against the prostaglandin E structure. The specific monoclonal antibody purified from ascites fluid was used for enzyme immunoassay, and as little as 30 pg of prostaglandin E1 and 100 pg of prostaglandin E2 were detected, which values are comparable to those obtained by radioimmunoassay. These results reveal that the hybridization technique is a reliable way to obtain prostaglandin E-specific antibody and that monoclonal antibodies can be valuable reagents for immunoassays.
将小鼠NS-1骨髓瘤细胞与用前列腺素E2牛血清白蛋白偶联物免疫的大鼠脾细胞融合后，从杂交骨髓瘤中获得了六种针对前列腺素E2的不同单克隆抗体。其中四种属于IgG2a亚类，另外两种分别是IgG2b和IgG2c。抗体对前列腺素E2的亲和力在5.8×10(6)-6.7×10(8) M-1范围内。交叉反应实验表明，一种单克隆抗体几乎只针对前列腺素E结构。从腹水中纯化的特异性单克隆抗体用于酶免疫测定，检测到低至30 pg的前列腺素E1和100 pg的前列腺素E2，这些值与放射免疫测定获得的值相当。这些结果表明，杂交技术是获得前列腺素E特异性抗体的可靠方法，单克隆抗体可以成为免疫测定中有价值的试剂。
