Pandey Kiran, Pradhan Asima, Bhagoliwal Ajay, Agarwal Asha, Mani Shweta
Department of Obstetrics and Gynaecology, G.S.V.M. Medical College Uttar Pradesh, Kanpur, Uttar Pradesh India.
Department of Physics & Centre for Laser and Photonics, IIT, Kanpur, Uttar Pradesh India.
J Obstet Gynaecol India. 2024 Aug;74(4):334-341. doi: 10.1007/s13224-023-01911-3. Epub 2023 Dec 19.
In 2020, the number of new cases of cervix uteri was 604,127, i.e., 3.1% of all cancers, and the number of deaths was 341,831 (3.3%) among both sexes. In vivo fluorescence spectroscopy is an emerging optical technology that offers promise for the diagnosis of disease & has the capability to quickly, noninvasively and quantitatively probe the biochemical and morphological changes that occur as tissue becomes dysplastic.
A cross-sectional observational study was conducted from December 2019 to September 2021 in the OBGY Department, UISEMH, in collaboration with optical imaging laboratory, BIOPHOTONICS, IIT Kanpur. A fabricated in-house fluorescence spectroscope consisting of a laser diode (405 nm) as light source and a miniature spectrometer is used to detect fluorescence signal from the sample. Patient's cervix was examined in the OPD, using an optical handheld probe, which functions on the principle of polarized fluorescence spectroscopy. The tissues were examined and classified on the basis of varying patterns of polarized spectroscopy (co-polarized, cross-polarized and co-minus cross-polarized light). The results were compared with that of cytological, colposcopy and histopathological findings and on various demographic variables.
In vivo handheld probe based on polarized fluorescence spectroscopy is an excellent screening technique. Co- and cross- polarized light has shown enhanced accuracy. Accuracy of co-minus cross-polarized light is poor. It is fast, noninvasive and quantitative and, with further developments, has the potential to become a regular screening tool in future.
2020年，子宫颈癌新发病例数为604,127例，占所有癌症病例的3.1%，男女死亡病例数为341,831例（占3.3%）。体内荧光光谱学是一种新兴的光学技术，有望用于疾病诊断，并且能够快速、非侵入性且定量地探测组织发育异常时发生的生化和形态学变化。
2019年12月至2021年9月，在UISEMH妇产科与坎普尔印度理工学院生物光子学光学成像实验室合作开展了一项横断面观察性研究。使用一种自制的荧光光谱仪，其由作为光源的激光二极管（405纳米）和微型光谱仪组成，用于检测来自样本的荧光信号。在门诊使用光学手持探头对患者的宫颈进行检查，该探头基于偏振荧光光谱原理工作。根据偏振光谱的不同模式（共偏振、交叉偏振和共减交叉偏振光）对组织进行检查和分类。将结果与细胞学、阴道镜检查和组织病理学检查结果以及各种人口统计学变量进行比较。
基于偏振荧光光谱的体内手持探头是一种出色的筛查技术。共偏振光和交叉偏振光显示出更高的准确性。共减交叉偏振光的准确性较差。它快速、非侵入性且定量，随着进一步发展，未来有可能成为一种常规筛查工具。
