荧光光谱法：宫颈癌研究的一种新方法。
Fluorescence spectroscopy: a new approach in cervical cancer.
作者信息
Pandey Kiran, Pradhan Asima, Agarwal Asha, Bhagoliwal Ajay, Agarwal Nidhi
机构信息
Department of Obstetrics & Gynaecology, G S V M Medical College, New Type IV/7, Medical College Campus, Kanpur, 208 002 India.
出版信息
J Obstet Gynaecol India. 2012 Aug;62(4):432-6. doi: 10.1007/s13224-012-0298-6. Epub 2012 Oct 23.
OBJECTIVE
To study the diagnostic potential of fluorescence spectroscopy and its comparison with different screening methods, including Pap smear and colposcopy, in detecting early cervical neoplasia.
METHOD
The study was conducted on patients with gynecological complaints. A full gynecological workup of the patients was done along with Pap smear and colposcopy. Cervical biopsy was done in suspected cases and fresh tissue was sent to IIT for spectroscopy.
RESULT
There is a definite increase in NADH fluorescence (67.4 %) and a decrease in collagen fluorescence (74 %) in dysplastic tissues. When epithelial fluorescence and stromal fluorescence are considered together, diagnostic accuracy is increased to 96.5 %.
CONCLUSION
The clinical diagnosis of cervical neoplasia by spectroscopic methods is potentially a reliable, fast, and cost-effective alternative to the conventional smear test which needs trained personnel for its interpretation. Research is still continuing to obtain a statistically significant cutoff value from in vitro studies and then use them for in vivo study.
目的
研究荧光光谱法在检测早期宫颈肿瘤形成中的诊断潜力，并将其与包括巴氏涂片和阴道镜检查在内的不同筛查方法进行比较。
方法
对有妇科症状的患者进行研究。对患者进行了全面的妇科检查，并进行了巴氏涂片和阴道镜检查。对疑似病例进行宫颈活检，将新鲜组织送至印度理工学院进行光谱分析。
结果
发育异常组织中烟酰胺腺嘌呤二核苷酸（NADH）荧光有明显增加（67.4%），胶原蛋白荧光减少（74%）。当同时考虑上皮荧光和基质荧光时，诊断准确率提高到96.5%。
结论
通过光谱法对宫颈肿瘤形成进行临床诊断，可能是一种可靠、快速且经济高效的替代传统涂片检查的方法，传统涂片检查需要训练有素的人员进行解读。研究仍在继续，以便从体外研究中获得具有统计学意义的临界值，然后将其用于体内研究。
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