Murwani Retno, Susilaningsih Neni, Ariyanto Diaza O, Ambariyanto Ambariyanto
Division of Natural Product Laboratory, Centre of Research and Services-Integrated Laboratory, Universitas Diponegoro, Semarang, Indonesia.
Department of Animal Science, Laboratory of Physiology and Biochemistry, Faculty of Animal and Agricultural Science, Universitas Diponegoro, Semarang, Indonesia.
Narra J. 2024 Aug;4(2):e853. doi: 10.52225/narra.v4i2.853. Epub 2024 Aug 6.
In vivo studies on the hazards of deep-fried foods were commonly done by feeding used-or heated-cooking oil to rats. The aim of this study was to determine the effect of feeding deep-fried in palm, olive, and coconut oils and the used frying oil on the blood biochemical profile of laboratory rats. An in vivo randomized control group study with pre-test and post-test was conducted. This study included healthy male Sprague-Dawley rats aged 2-3 months and weighing 100-200 grams. After acclimatization, the rats were randomly assigned to seven groups, which were: (1) regular diet (control diet); (2) diet of deep-fried in 5× used palm oil (-in-used-Po); (3) diet of deep-fried in 5× used coconut oil (-in-used-Co); (4) diet of deep-fried in 5× used olive oil (-in-used-Oo); (5) diet of 5× used palm oil (Used-Po); (6) diet of 5× used coconut oil (Used-Co); and (7) diet of 5× used olive oil (Used-Oo). Each rat received 15 grams of a treatment diet daily and blood samples were collected after four weeks for a complete blood count and serum biochemistry analysis. The results showed that the final body weight and the weight gain of -in-used-Po, -in-used-Co, -in-used-Oo group, and Used-Po groups increased significantly compared to the control, Used-Co, and Used-Oo groups. However, there was a significant increase in serum tumor necrosis factor-alpha (TNF-α) in the Used-Co and Used-Oo groups (<0.05), suggesting the used oil's detrimental effect. The Used-Co and Used-Oo were the only two groups whose creatinine increased significantly (<0.05). Subsequently, only the Used-Oo group had a significantly increased malondialdehyde (MDA) level compared to all groups (<0.05). These results prove that the effect of feeding fried food differs from used oils. Feeding used oil did not reflect the consumption of fried foods as part of the whole diet and generally resulted in more harmful effects. This is the first study to report an in vivo rat feeding study of deep-fried and the used oil as part of the diet.
关于油炸食品危害的体内研究通常是通过给大鼠喂食用过的或加热过的食用油来进行的。本研究的目的是确定喂食用棕榈油、橄榄油和椰子油油炸的食品以及用过的煎炸油对实验大鼠血液生化指标的影响。进行了一项带有预测试和后测试的体内随机对照组研究。本研究纳入了2至3个月大、体重100至200克的健康雄性斯普拉格-道利大鼠。适应环境后，将大鼠随机分为七组，分别是：(1)常规饮食（对照饮食）；(2)用5倍量用过的棕榈油炸制的食品饮食（-in-used-Po）；(3)用5倍量用过的椰子油炸制的食品饮食（-in-used-Co）；(4)用5倍量用过的橄榄油炸制的食品饮食（-in-used-Oo）；(5)5倍量用过的棕榈油饮食（Used-Po）；(6)5倍量用过的椰子油饮食（Used-Co）；以及(7)5倍量用过的橄榄油饮食（Used-Oo）。每只大鼠每天接受15克治疗饮食，四周后采集血液样本进行全血细胞计数和血清生化分析。结果显示，与对照组、Used-Co组和Used-Oo组相比，-in-used-Po组、-in-used-Co组、-in-used-Oo组和Used-Po组的最终体重和体重增加显著。然而，Used-Co组和Used-Oo组的血清肿瘤坏死因子-α（TNF-α）显著升高（<0.05），表明用过的油具有有害影响。Used-Co组和Used-Oo组是仅有的两个肌酐显著升高的组（<0.05）。随后，与所有组相比，只有Used-Oo组的丙二醛（MDA）水平显著升高（<0.05）。这些结果证明，喂食油炸食品的影响与用过的油不同。喂食用过的油并不能反映油炸食品作为整个饮食一部分的食用情况，并且通常会导致更多有害影响。这是第一项报告将油炸食品和用过的油作为饮食一部分进行体内大鼠喂养研究的研究。
