Al-Groom Rania M, Ali Rand Raid Mahmoud, Shaqra Qasem M Abu
Rania M. Al-Groom, Department of Medical Laboratory Science, Faculty of Allied Medical Sciences, Zarqa University, Zarqa, Jordan.
Rand Raid Mahmoud Ali, Faculty of Graduate Studies, Al-Balqa Applied University, Salt, Jordan.
Pak J Med Sci. 2024 Sep;40(8):1619-1624. doi: 10.12669/pjms.40.8.9811.
BACKGROUND & OBJECTIVES: Genotypic identification of the etiologic agents of vaginal candidiasis (VC) is of significance in epidemiologic studies and in the establishment of adequate treatment protocol. The aim of this study was to determine the antifungal susceptibility and gene diversity of isolated from a group of Jordanian women with VC.
A total of 312 isolates of candida species, recovered from women with vaginal candidiasis who attended gynecology clinics affiliated to three major private hospitals in Amman over a period of five months (July 2020 to December 2020) were included in this study. The isolated Candida were characterized by phenotypic and genotypic means. Genotypic studies were performed using specific PCR primers of the rDNA and RPS genes. Susceptibility testing of all isolates was conducted following the National Committee for Clinical Laboratory Standards and E-test strips.
was the most dominant . that caused VC among the studied population. isolates were found to be of three different subtypes at the 25S rDNA gene. All isolates belonged to genotypes A, B and C while genotypes D and E were not detected. The diversity of was higher on the basis of RPS region where the use of two markers (P-I and P-II) resulted in the identification of nine distinct subtypes. The sensitivity testing revealed variations in the susceptibility of various genotypes to different antifungal drugs. Genotype A isolates were more susceptible to fluconazole, flucytosine and ketoconazole than genotypes B and C.
incriminated as etiologic agents of vaginitis among Jordanian women exhibited relationship between various genotypes and antifungal drugs.
阴道念珠菌病（VC）病原体的基因型鉴定在流行病学研究及制定适当的治疗方案中具有重要意义。本研究旨在确定从一组患VC的约旦女性中分离出的念珠菌的抗真菌药敏性及基因多样性。
本研究纳入了在五个月期间（2020年7月至2020年12月）从安曼三家主要私立医院附属妇科诊所就诊的患阴道念珠菌病的女性中分离出的312株念珠菌。分离出的念珠菌通过表型和基因型方法进行鉴定。使用rDNA和RPS基因的特异性PCR引物进行基因研究。按照美国国家临床实验室标准委员会和E-test试纸对所有分离株进行药敏试验。
在所研究人群中，白色念珠菌是导致VC的最主要病原体。在25S rDNA基因处发现分离株有三种不同亚型。所有分离株均属于A、B和C基因型，未检测到D和E基因型。基于RPS区域，白色念珠菌的多样性更高，使用两个标记（P-I和P-II）鉴定出九个不同的白色念珠菌亚型。药敏试验显示不同基因型对不同抗真菌药物的敏感性存在差异。A基因型分离株比B和C基因型对氟康唑、氟胞嘧啶和酮康唑更敏感。
在约旦女性中被认定为阴道炎病原体的白色念珠菌显示出不同基因型与抗真菌药物之间的关系。
