Nadeem Amna, Sadiqa Ayesha, Saeed Muniza
Amna Nadeem, M.Phil. Department of Physiology, CMH Lahore Medical College and Institute of Dentistry, Lahore, Pakistan. National University of Medical Sciences, Rawalpindi, Pakistan.
Ayesha Sadiqa, M.Phil. Ph.D. Department of Physiology, CMH Lahore Medical College and Institute of Dentistry, Lahore, Pakistan. National University of Medical Sciences, Rawalpindi, Pakistan.
Pak J Med Sci. 2024 Sep;40(8):1786-1790. doi: 10.12669/pjms.40.8.9308.
To determine the effect of Vitamin-D-supplementation on glycemic parameters: glucose levels in blood, insulin, HbA1c, HOMA-IR, and adiponectin in women with gestational diabetes.
An experimental study was executed at PGMI/LGH of Lahore from June 2020 to June 2021, with 34 Vitamin-D-deficient women who had gestational diabetes (20-26 weeks). All were aged between 21-32 years, randomly and equally divided into controls and cases. Cases received 200,000 IU Vitamin-D-dose. Fasting blood was collected before as well as after treatment from each participant. Spectrophotometry and peroxidase method were used to estimate HbA1c and glucose concentrations respectively. Insulin, adiponectin, and Vitamin-D were assessed by ELISA. To verify data normality, the Shapiro-Wilk test was applied and to prove group comparison Mann-Whitney U, Wilcoxon signed-rank, and Sample-t tests were used via IBM-SPSS version-21.
No difference in blood glucose was found between controls and cases before treatment (p=0.858), while post-treatment, significant reduction found in cases (p=0.019). Before treatment, no difference was noticed in insulin levels of both groups (p=0.44), however, post-treatment, a significant decline was expressed in cases (p=0.001). No difference was found in HOMA-IR between controls and cases before treatment (p=0.14) but post-treatment, significant reduction was observed in cases (p=0.001). Non-significant difference was noted in HbA1c before (p=0.664) and after (p=0.169) treatment in both groups. Non-significant upsurge in adiponectin was observed in cases before (p=0.544) and after (p=0.194) treatment.
Vitamin-D supplementation significantly improves glycemic control in gestational diabetic women, however, its effect on adiponectin was non-significant.
确定补充维生素D对妊娠糖尿病女性血糖参数的影响，这些参数包括血糖水平、胰岛素、糖化血红蛋白（HbA1c）、胰岛素抵抗指数（HOMA-IR）和脂联素。
2020年6月至2021年6月在拉合尔的PGMI/LGH进行了一项实验研究，研究对象为34名患有妊娠糖尿病（孕周20 - 26周）且维生素D缺乏的女性。所有女性年龄在21 - 32岁之间，随机且平均分为对照组和病例组。病例组接受200,000 IU的维生素D剂量。在治疗前后从每位参与者采集空腹血样。分别采用分光光度法和过氧化物酶法测定HbA1c和葡萄糖浓度。通过酶联免疫吸附测定法（ELISA）评估胰岛素、脂联素和维生素D。为验证数据正态性，应用夏皮罗-威尔克检验（Shapiro-Wilk test），并通过IBM-SPSS 21版软件使用曼-惠特尼U检验（Mann-Whitney U）、威尔科克森符号秩检验（Wilcoxon signed-rank）和样本t检验（Sample-t tests）进行组间比较。
治疗前对照组和病例组的血糖无差异（p = 0.858），而治疗后病例组血糖显著降低（p = 0.019）。治疗前两组胰岛素水平无差异（p = 0.44），但治疗后病例组胰岛素水平显著下降（p = 0.001）。治疗前对照组和病例组的胰岛素抵抗指数（HOMA-IR）无差异（p = 0.14），但治疗后病例组显著降低（p = 0.001）。两组治疗前（p = 0.664）和治疗后（p = 0.169）的糖化血红蛋白（HbA1c）无显著差异。病例组治疗前（p = 0.544）和治疗后（p = 0.194）的脂联素无显著升高。
补充维生素D可显著改善妊娠糖尿病女性的血糖控制，然而，其对脂联素的影响不显著。
