Jin Yihua, Geng Zhimin, Lin Kun, Gu Xinyu, Feng Xiwei, Fu Songling, Wang Wei, Xie Chunhong, Wang Yujia, Gong Fangqi
Department of Cardiology, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, People's Republic of China.
Pediatric Cardiovascular Diseases Laboratory, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, Zhejiang, People's Republic of China.
J Inflamm Res. 2024 Sep 9;17:6193-6201. doi: 10.2147/JIR.S468472. eCollection 2024.
Kawasaki disease (KD) is an acute systemic vasculitis that is associated with dysregulated immune responses. Monocytes play a central role in innate immunity. Our previous single-cell RNA sequencing of peripheral blood mononuclear cells (PBMC) revealed a new subset of monocytes in children with KD called L-Selectin classical monocytes (SELL CM). Therefore, we aimed to investigate the correlation between KD and SELL CM.
Peripheral blood samples were collected from 81 KD patients, 18 febrile patients and 36 healthy children before treatment. Among them, ten KD patients were followed up, and samples were obtained before and after intravenous immunoglobulin (IVIG) treatment. Analysis of SELL CM was performed using flow cytometry. Additionally, ROC curve analysis was conducted to assess the diagnostic value of SELL CM for KD.
Classical monocytes (CM) expressed the highest levels of L-selectin in children with KD. The ratio of SELL CM in CM was significantly higher in KD patients than in febrile and healthy children. Following IVIG treatment, the ratio of SELL CM in CM showed a downward trend. The receiver operating characteristic (ROC) curve analysis (the area under the curve, AUC = 0.71) indicated the potential diagnostic value of SELL CM in KD. The correlation analysis suggested that SELL CM may serve as a new clinical index for patients with KD.
In KD, the ratio of SELL CM in CM significantly increases during the acute phase, which may become a potential biomarker and help facilitate KD diagnosis based on clinical features.
川崎病（KD）是一种与免疫反应失调相关的急性全身性血管炎。单核细胞在固有免疫中起核心作用。我们之前对外周血单核细胞（PBMC）进行的单细胞RNA测序揭示了KD患儿中一种新的单核细胞亚群，称为L-选择素经典单核细胞（SELL CM）。因此，我们旨在研究KD与SELL CM之间的相关性。
在治疗前，从81例KD患者、18例发热患者和36例健康儿童中采集外周血样本。其中，对10例KD患者进行随访，并在静脉注射免疫球蛋白（IVIG）治疗前后采集样本。使用流式细胞术分析SELL CM。此外，进行ROC曲线分析以评估SELL CM对KD的诊断价值。
在KD患儿中，经典单核细胞（CM）表达的L-选择素水平最高。KD患者中CM内SELL CM的比例显著高于发热患者和健康儿童。IVIG治疗后，CM内SELL CM的比例呈下降趋势。受试者工作特征（ROC）曲线分析（曲线下面积，AUC = 0.71）表明SELL CM在KD中具有潜在诊断价值。相关性分析表明，SELL CM可能作为KD患者的一项新的临床指标。
在KD中，急性期CM内SELL CM的比例显著增加，这可能成为一种潜在的生物标志物，并有助于根据临床特征促进KD的诊断。
