Optimization of Cultivation Conditions of Native Microalga and Evaluation of Lipids for Enhanced Biodiesel Production.

Biofuels are considered to be among the primary alternatives to the use of fossil fuels. These fuels, made from feedstock or waste raw materials, have the advantage of being renewable and contributing much less to global warming. Microalgae are a promising biodiesel source. Microalgae, unlike traditional crops that are now used to make commercialized biodiesel, may be grown on non-agricultural land and has a greater capacity for growth and yield. Cultivation has been considered as a critical stage in the generation of biofuels. The goal of the present study is to learn that has a potential for biodiesel production in the near future. Optimization studies revealed that BG-11 medium, temperature of 25 °C, pH 7.0, glucose and sucrose (as carbon sources), static condition (for lipid accumulation) & shaking condition (for biomass yield), cultivation days of 18, 21, and 24 day, NaNO dosing of 1.0 mM followed by 0.8 mM (on 5th day of cultivation), 3% yeast extract dosing, 3000 lx light intensity, photoperiod cycles of 24L/0D (for biomass yield) and 18L/6D (for lipid production) and 10 mM concentration of NaCl (salinity stress) can be regarded as best suited physio-biochemical parameters for efficient biomass and lipid yield from . FTIR indicated presence of various stretching of carbohydrates and lipids that again is supporting biodiesel production capability of . SEM showed that cells of under stress conditions became fragmented separated from coenobium and were not so compactly arranged. Present optimization studies along with Nile red fluorescence, FTIR and SEM revealed that could be a suitable candidate to produce good quality biofuel and that also in stress conditions.