Neeva Nahla Islam, Zafrin Nahida, Jhuma Azima Aktar, Chowdhury Sufia Khanom, Fatema Kaniz, Rifat Tafhim Ahmed
Department of Microbiology, MARKS Medical College and Hospital, Dhaka, Bangladesh.
Department of Medicine, Sylhet MAG Osmani Medical College, Sylhet, Bangladesh.
Indian J Microbiol. 2024 Sep;64(3):1025-1034. doi: 10.1007/s12088-024-01216-7. Epub 2024 Feb 29.
One of the most prevalent infectious diseases identified in both communities and hospitalized patients is urinary tract infection (UTI). is evolved into a clinically pertinent uropathogen due to its evolving resistance to multiple antimicrobial agentsThis study, detects antimicrobial susceptibility patterns of species and molecular detection of from patients with urinary tract infections. In this cross-sectional observational study, 165 urine samples were obtained from clinically diagnosed patients with UTIs of different ages and gender. species were identified by standard microbiological procedure and PCR (by using species-specific primers for ). A modified Kirby Bauer Disc diffusion method was used to identify the antimicrobial susceptibility pattern following Clinical and Laboratory Standards Institute (CLSI) guidelines. Out of 165 urine samples, 134 samples yielded positive cultures. species were isolated from 23 (17.1%) urine samples. Among all , 16 (69.6%) isolates were . , detected by PCR assay. A higher (30.4%) proportion of -positive patients were from the age group 48-57 years and female patients (78.2%) had a higher prevalence. Enterococcal infection was found in 56.5% of non-catheterized patients and 43.5% of catheterized patients. Vancomycin and linezolid (78.3%) and meropenem (73.9%) sensitivity was prevalent among all species. They showed 100% resistance towards ceftriaxone, cefixime 95.7%, cefuroxime 91.3%, azithromycin 82.6%. This research indicated the occurrence of species and the advent of multidrug-resistant in patients with UTIs. Routine speciation and antimicrobial susceptibility testing of in various clinical samples is encouraged.
在社区和住院患者中发现的最常见的传染病之一是尿路感染（UTI）。由于其对多种抗菌药物的耐药性不断演变，它已演变成一种临床相关的尿路病原体。本研究检测了尿路感染患者中该菌种的抗菌药敏模式并进行分子检测。在这项横断面观察研究中，从不同年龄和性别的临床诊断为UTI的患者中获取了165份尿液样本。通过标准微生物学程序和PCR（使用该菌种特异性引物）鉴定菌种。采用改良的 Kirby Bauer 纸片扩散法按照临床和实验室标准研究所（CLSI）指南确定抗菌药敏模式。在165份尿液样本中，134份样本培养结果为阳性。从23份（17.1%）尿液样本中分离出该菌种。在所有分离株中，16份（69.6%）为该菌种。通过PCR检测发现。48 - 57岁年龄组中该菌种阳性患者比例较高（30.4%），女性患者患病率较高（78.2%）。在非导尿患者中56.5%发现肠球菌感染，导尿患者中43.5%发现感染。在所有该菌种中，万古霉素和利奈唑胺（78.3%）以及美罗培南（73.9%）敏感性普遍。它们对头孢曲松耐药率达100%，头孢克肟为95.7%，头孢呋辛为91.3%，阿奇霉素为82.6%。本研究表明UTI患者中该菌种的出现以及多重耐药该菌种情况的出现。鼓励对各种临床样本中的该菌种进行常规菌种鉴定和抗菌药敏试验。
