在孟加拉国从人类临床标本中分离出介导万古霉素耐药的屎肠球菌（ST1912/CC116）和粪肠球菌（ST80/CC17）、携带cfr的耐利奈唑胺屎肠球菌（ST32、ST1902） 。
Isolation of -Mediated Vancomycin-Resistant (ST1912/CC116) and (ST80/CC17), -Positive Linezolid-Resistant (ST32, ST1902) from Human Clinical Specimens in Bangladesh.
作者信息
Roy Sangjukta, Aung Meiji Soe, Paul Shyamal Kumar, Khan Md Nazmul Alam, Nasreen Syeda Anjuman, Hasan Muhammad Saiful, Haque Nazia, Barman Tridip Kanti, Khanam Jobyda, Sathi Fardousi Akter, Paul Shashwata, Ali Mohammad Ibrahim, Kobayashi Nobumichi
机构信息
Department of Microbiology, Mymensingh Medical College, Mymensingh 2200, Bangladesh.
Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan.
出版信息
Antibiotics (Basel). 2025 Mar 4;14(3):261. doi: 10.3390/antibiotics14030261.
BACKGROUND/OBJECTIVES: is one of the major nosocomial pathogens. The present status of antimicrobial resistance determinants and virulence factors was analyzed for current causing infectious diseases in Bangladesh.
METHODS
Clinical isolates of recovered from various specimens in a tertiary care hospital were analyzed. Antimicrobial susceptibility was measured by a broth microdilution test, and resistance genes/virulence factors were detected by uniplex/multiplex PCR, along with sequencing analysis as required. The sequence type (ST) of and was identified based on a multilocus sequence typing (MLST) scheme.
RESULTS
For a one-year period, a total of 143 isolates (135 , 7 , and 1 ) were collected. Although all isolates were susceptible to penicillin, high resistance rates were noted against erythromycin (87%) and levofloxacin (62%). High-level resistance to gentamicin was detected in 30% of and 86% of . Vancomycin resistance due to was identified in one isolate each of (ST1912, CC116) and (ST80, CC17). Three isolates (2.2%) with ST32 or ST1902 were resistant to linezolid, harboring .
CONCLUSIONS
The present study identifies the vancomycin-resistant harboring from humans in Bangladesh and shows the potential spread of in multiple lineages of .
背景/目的：[病原体名称]是主要的医院感染病原体之一。分析了孟加拉国当前引起传染病的[病原体名称]的抗菌药物耐药决定因素和毒力因子的现状。
方法
对一家三级护理医院从各种标本中分离出的[病原体名称]临床分离株进行分析。采用肉汤微量稀释试验测定抗菌药物敏感性，通过单重/多重聚合酶链反应（PCR）检测耐药基因/毒力因子，并根据需要进行测序分析。根据多位点序列分型（MLST）方案确定[病原体名称]的序列类型（ST）。
结果
在一年时间里，共收集了143株分离株（135株[病原体名称]、7株[相关病原体名称1]和1株[相关病原体名称2]）。虽然所有[病原体名称]分离株对青霉素敏感，但对红霉素（87%）和左氧氟沙星（62%）的耐药率较高。在30%的[病原体名称1]和86%的[病原体名称2]中检测到对庆大霉素的高水平耐药。在[病原体名称1]（ST1912，CC116）和[病原体名称2]（ST80，CC17）的各一株分离株中鉴定出由[相关基因名称]导致的万古霉素耐药。三株序列类型为ST32或ST1902的[病原体名称]分离株（2.2%）对利奈唑胺耐药，携带[相关基因名称]。
结论
本研究在孟加拉国人类中鉴定出携带[相关基因名称]的万古霉素耐药[病原体名称]，并显示[病原体名称]在多个谱系中有潜在传播。
Zotero 插件