IM17 水溶性胞外多糖的特性分析
Characterization of Water-Soluble Extracellular Polysaccharide from IM17.
作者信息
Genc Berna, Tunç Merve Tuğçe, Adiguzel Ahmet
机构信息
Department of Genetics and Bioengineering, Faculty of Engineering and Life Sciences, Gumushane University, 29100 Gumushane, Turkey.
Department of Food Engineering, Faculty of Engineering and Life Sciences, Gumushane University, 29100 Gumushane, Turkey.
出版信息
Indian J Microbiol. 2024 Sep;64(3):973-982. doi: 10.1007/s12088-023-01178-2. Epub 2023 Dec 29.
The production of the extracellular polysaccharide from the thermophilic bacterium was carried out in the study. The polysaccharide was isolated and characterized by means of GC-MS, FT-IR, DSC, and XRD analyses. The rheological, foaming, and emulsifying properties of the polysaccharide were determined. Using a sucrose-rich medium, 27.1 mg dried EPS/100 mL was obtained with 94% carbohydrate and 1.5% protein. The monosaccharide profile of water-soluble EPS-IM17 was composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose. The foaming capacity and stability of EPS-IM17 were 26.67% (± 4.71) and 40.01% (± 4.95), respectively, and the foaming stability was not affected by time. The emulsification index of EPS-IM17 was 64.54 (± 8.71) and decreased to 38.47 (± 10.44) after 24 h. EPS-IM17 had a crystalline structure. Solutions at different concentrations (10, 20, 40 mgmL) showed pseudoplastic behavior. In conclusion, this report could be a lead study for the use of extracellular polysaccharide for different applications.
本研究开展了嗜热细菌胞外多糖的制备工作。通过气相色谱-质谱联用（GC-MS）、傅里叶变换红外光谱（FT-IR）、差示扫描量热法（DSC）和X射线衍射（XRD）分析对该多糖进行了分离和表征。测定了该多糖的流变学、发泡和乳化性能。使用富含蔗糖的培养基，获得了27.1毫克干燥胞外多糖/100毫升，其中碳水化合物含量为94%，蛋白质含量为1.5%。水溶性胞外多糖-IM17的单糖组成包括鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖。胞外多糖-IM17的发泡能力和稳定性分别为26.67%（±4.71）和40.01%（±4.95），且发泡稳定性不受时间影响。胞外多糖-IM17的乳化指数为64.54（±8.71），24小时后降至38.47（±10.44）。胞外多糖-IM17具有晶体结构。不同浓度（10、20、40毫克/毫升）的溶液表现出假塑性行为。总之，本报告可为将胞外多糖用于不同应用提供先导性研究。
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