Patel Sandip S, Patel Arun C, Mohapatra Sushil K, Chauhan Harshad C, Sharma Kishan K, Shrimali Mehul D, Raval Samir H, Prajapati Bhavesh I
Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar- Dantiwada, Banaskantha, Gujarat 385506 India.
Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar- Dantiwada, Banaskantha, Gujarat 385506 India.
Indian J Microbiol. 2024 Sep;64(3):917-926. doi: 10.1007/s12088-023-01132-2. Epub 2024 Jan 17.
In the present study, a total of 102 samples were collected from chickens of different flocks, died due to suspected colibacillosis. Bacteriological and PCR methods were applied to detect avian pathogenic (APEC). Phenotypic antimicrobial resistance (AMR) was determined by disk diffusion method. Extended spectrum beta lactamases (ESBL) detection was carried out via PCR by targeting TEM, SHV, and CTX-M groups 1, 2, and 9. Genes of eight virulence factors and class I integrons were also detected by PCR using gene specific primers. Culture, microscopic, biochemical tests and PCR recognised 69/102 (67.64%) samples as . Phenotypic AST revealed higher resistance against fluoroquinolone antibiotics, ., enrofloxacin (72.46%), levofloxacin (69.56%) & ciprofloxacin (66.66%), followed by amoxyclav (63.77%) and tetracycline (59.42%). Six isolates were found as pan-drug-resistant . A total of 48 (69.56%) and 7 (10.14%) isolates were positive for the presence of TEM and CTX-M-G9 genes, respectively, whereas 2 (2.90%) isolates each were found positive for SHV, and CTX-M-G1 genes. Among APEC associated virulence genes, (79.71%) was the most predominant, followed by (50.72%), (30.43%), (26.08%), (23.18%), vat (8.69%) and -1 (1.44%). Thirty-two isolates harboured class I integrons, either with or without ESBL genes. Conclusively, the isolates under study showed pan and multiple-drug resistance, specifically against fluoroquinolone drugs. ESBL production was mediated principally through and CTX-M-G9. Multiple virulence factors, toxins, and carriage & spread factor render these as zoonotically potential pathogens for humans.
The online version contains supplementary material available at 10.1007/s12088-023-01132-2.
在本研究中，共从不同鸡群中收集了102份疑似死于大肠杆菌病的鸡的样本。采用细菌学和PCR方法检测禽致病性大肠杆菌（APEC）。通过纸片扩散法测定表型抗菌药物耐药性（AMR）。通过针对TEM、SHV以及CTX-M 1、2和9组进行PCR检测超广谱β-内酰胺酶（ESBL）。还使用基因特异性引物通过PCR检测了8种毒力因子和I类整合子的基因。培养、显微镜检查、生化试验和PCR鉴定出69/102（67.64%）的样本为……。表型药敏试验显示对氟喹诺酮类抗生素耐药性较高，例如恩诺沙星（72.46%）、左氧氟沙星（69.56%）和环丙沙星（66.66%），其次是阿莫西林克拉维酸（63.77%）和四环素（59.42%）。发现6株分离株为泛耐药……。共有48株（69.56%）和7株（10.14%）分离株分别对TEM和CTX-M-G9基因呈阳性，而各有2株（2.90%）分离株对SHV和CTX-M-G1基因呈阳性。在与APEC相关的毒力基因中，……（79.71%）最为主要，其次是……（50.72%）、……（30.43%）、……（26.08%）、……（23.18%）、vat（8.69%）和……-1（1.44%）。32株分离株携带I类整合子，有或没有ESBL基因。总之，所研究的分离株显示出泛耐药和多重耐药，特别是对氟喹诺酮类药物。ESBL的产生主要由……和CTX-M-G9介导。多种毒力因子、毒素以及携带和传播因子使这些分离株成为对人类具有人畜共患病潜力的病原体。
在线版本包含可在10.1007/s12088-023-01132-2获取的补充材料。
