Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan.
Methods Mol Biol. 2025;2856:157-176. doi: 10.1007/978-1-0716-4136-1_9.
Hi-C and 3C-seq are powerful tools to study the 3D genomes of bacteria and archaea, whose small cell sizes and growth conditions are often intractable to detailed microscopic analysis. However, the circularity of prokaryotic genomes requires a number of tricks for Hi-C/3C-seq data analysis. Here, I provide a practical guide to use the HiC-Pro pipeline for Hi-C/3C-seq data obtained from prokaryotes.
Hi-C 和 3C-seq 是研究细菌和古菌三维基因组的强大工具，这些微生物的细胞体积小，生长条件往往难以进行详细的显微镜分析。然而，原核生物基因组的环状结构需要一些技巧来进行 Hi-C/3C-seq 数据分析。在这里，我提供了一个实用指南，介绍如何使用 HiC-Pro 管道来处理从原核生物中获得的 Hi-C/3C-seq 数据。
