Haas W, Kisielow P
Eur J Immunol. 1985 Aug;15(8):751-5. doi: 10.1002/eji.1830150802.
Fluorescein-specific cytolytic hybrids were obtained by fusion of an interleukin 2 (IL2)-dependent murine cytotoxic T lymphocyte clone with the thymoma BW5147. The hybrid cells grow and retain cytolytic activity in medium with recombinant IL2. In medium without IL2 rare variants can be selected which grow in the absence of exogenous IL2; these variants are not cytolytic. Anti-IL2 receptor antibodies bind to and inhibit the growth of the cytolytic hybrids while they do not bind to and do not inhibit the growth of the variants. The cytolytic hybrids as well as the non-cytolytic variants produce interferon gamma in response to antigen. These findings indicate that the noncytolytic IL2-dependent variants lost IL2 receptors but not antigen receptors.
通过将白细胞介素2（IL2）依赖性小鼠细胞毒性T淋巴细胞克隆与胸腺瘤BW5147融合，获得了荧光素特异性溶细胞杂种细胞。杂种细胞在含有重组IL2的培养基中生长并保留溶细胞活性。在不含IL2的培养基中，可以选择在没有外源性IL2的情况下生长的罕见变体；这些变体不具有溶细胞活性。抗IL2受体抗体与溶细胞杂种细胞结合并抑制其生长，而它们不与变体结合也不抑制变体的生长。溶细胞杂种细胞以及非溶细胞变体在受到抗原刺激时会产生γ干扰素。这些发现表明，非溶细胞性IL2依赖性变体失去了IL2受体，但没有失去抗原受体。
