Viktorov I V, Lyzhin A A, Shashkova N A
Biull Eksp Biol Med. 1985 May;99(5):632-4.
An original method of cultivation of reaggregated brain cells with the aid of high-speed portable mini-rollers is described. The mini-roller consists of parallel rollers and an electric motor rotating the flasks at a speed of 60 to 70 rpm. The Moscona technique was used for preparing brain cell suspensions. During cultivation of the suspension of dissociated cells in high-speed mini-rollers, reaggregates with an internal organotypic structure were obtained. The method suggested provides stable and reproducible results.
描述了一种借助高速便携式微型滚筒培养重新聚集的脑细胞的原始方法。微型滚筒由平行滚筒和以60至70转/分钟的速度旋转培养瓶的电动马达组成。使用莫斯孔纳技术制备脑细胞悬液。在高速微型滚筒中培养解离细胞悬液的过程中，获得了具有内部器官型结构的重新聚集物。所建议的方法提供了稳定且可重复的结果。
