Li Jia-Jun, Lu Ting-Ting, Mo Wen-Jing, Yu Hai-Xia, Li Kai-Jiang, Huang Xing, Fan Zhi-Yi, He Xin-Hua, Luo Cong
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning 530004, Guangxi, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning 530004, Guangxi, China; Guangxi Key Laboratory of High-Quality Formation and Utilization of Dao-di Herbs, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China.
Int J Biol Macromol. 2024 Sep 14;280(Pt 1):135669. doi: 10.1016/j.ijbiomac.2024.135669.
Phosphatidylethanolamine binding protein (PEBP) family plays important roles in multiple developmental processes in plants. In this study, a total of 11 PEBP gene family members were identified from the mango (Mangifera indica L.) genome, and these proteins were divided into three subfamilies based on their phylogenetic relationships: TERMINAL FLOWER 1 (TFL1)-like, MOTHER OF FT AND TFL (MFT)-like, and FLOWERING LOCUS T (FT)-like. Expression analysis revealed that MiFT1a, MiFT1b and MiFT2 were expressed mainly in leaves, whereas MiFT3 and MiFT4 were expressed mainly in embryos. The overexpression of MiFTs significantly promoted early flowering under both long- and short-day conditions. Interestingly, it still significantly promoted early flowering at 16 °C and 28 °C, with MiFT1a exhibiting the most significant, followed by MiFT1b and MiFT2. Additionally, the expression level of MiFT3 is related to the embryonic development of mango. Further studies revealed that overexpression of MiFT3 inhibited seed germination in transgenic Arabidopsis lines. In addition, the MiFT1a and MiFT1b transgenic lines did not respond to abiotic stress, while MiFT2, MiFT3 and MiFT4 enhanced resistance to salt or drought stress in Arabidopsis. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays revealed that MiFTs can interact with flower related and multiple stress proteins, such as bZIP protein (MiFD), 14-3-3 protein, zinc finger protein (MiZFP4), RING zinc-finger protein (MiRZFP34), and phosphatase 2C (MiPP2C25A and MiPP2C25B). These results indicate that FT subfamily not only regulates flowering but also participates in stress response, but there are differences in the function among these genes.
磷脂酰乙醇胺结合蛋白（PEBP）家族在植物的多个发育过程中发挥着重要作用。在本研究中，从芒果（Mangifera indica L.）基因组中总共鉴定出11个PEBP基因家族成员，这些蛋白质根据其系统发育关系被分为三个亚家族：类TERMINAL FLOWER 1（TFL1）、类MOTHER OF FT AND TFL（MFT）和类FLOWERING LOCUS T（FT）。表达分析表明，MiFT1a、MiFT1b和MiFT2主要在叶片中表达，而MiFT3和MiFT4主要在胚中表达。MiFTs的过表达在长日照和短日照条件下均显著促进早花。有趣的是，在16℃和28℃时它仍能显著促进早花，其中MiFT1a表现最为显著，其次是MiFT1b和MiFT2。此外，MiFT3的表达水平与芒果的胚胎发育有关。进一步研究表明，MiFT3的过表达抑制了转基因拟南芥株系的种子萌发。此外，MiFT1a和MiFT1b转基因株系对非生物胁迫无响应，而MiFT2、MiFT3和MiFT4增强了拟南芥对盐或干旱胁迫的抗性。酵母双杂交（Y2H）和双分子荧光互补（BiFC）分析表明，MiFTs可与花相关蛋白和多种胁迫蛋白相互作用，如bZIP蛋白（MiFD）、14-3-3蛋白、锌指蛋白（MiZFP4）、RING锌指蛋白（MiRZFP34）和磷酸酶2C（MiPP2C25A和MiPP2C25B）。这些结果表明，FT亚家族不仅调控开花，还参与胁迫响应，但这些基因在功能上存在差异。
