Paradowska Edyta, Haręża Daria A, Kania Katarzyna D, Jarych Dariusz, Wilczyński Miłosz, Malinowski Andrzej, Kawecka Monika, Nowak Mateusz, Wilczyński Jacek R
Laboratory of Virology, The Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa St., 93-232, Lodz, Poland.
BioMedChem Doctoral School of the University of Lodz and the Institutes of the Polish Academy of Sciences in Lodz, Lodz, Poland.
Sci Rep. 2024 Sep 16;14(1):21602. doi: 10.1038/s41598-024-72814-0.
Human papillomaviruses (HPVs) and herpesviruses are detected in patients with epithelial ovarian cancer (EOC). We sought to analyze the prevalence of HPV's 16 and 18, cytomegalovirus (CMV), and Epstein-Barr virus (EBV) DNA in peripheral blood, ovarian, and fallopian tube (FT) tissue samples collected from 97 EOC patients, including 71 cases of high-grade serous ovarian carcinoma (HGSOC), and from 60 women with other tumors or non-neoplastic gynecological diseases. DNA isolates were analyzed by PCR methods, including droplet digital PCR. The results demonstrate that (1) HPV16 DNA has been detected in one-third of the FT and tumor samples from EOCs; (2) the prevalence and quantity of HPV16 DNA were significantly higher in FT samples from HGSOCs, non-HGSOCs, and ovarian metastases than in those from non-neoplastic diseases; (3) CMV and EBV have been detected in approximately one-seventh of EOC samples. The results suggest that HPV16 might be a potential risk factor for EOC development.
在上皮性卵巢癌（EOC）患者中检测到了人乳头瘤病毒（HPV）和疱疹病毒。我们试图分析从97例EOC患者（包括71例高级别浆液性卵巢癌（HGSOC））以及60例患有其他肿瘤或非肿瘤性妇科疾病的女性中采集的外周血、卵巢和输卵管（FT）组织样本中HPV 16和18、巨细胞病毒（CMV）和爱泼斯坦-巴尔病毒（EBV）DNA的流行情况。通过包括液滴数字PCR在内的PCR方法对DNA分离物进行分析。结果表明：（1）在三分之一的EOC患者的FT和肿瘤样本中检测到了HPV16 DNA；（2）HGSOC、非HGSOC和卵巢转移瘤的FT样本中HPV16 DNA的流行率和数量显著高于非肿瘤性疾病的样本；（3）在大约七分之一的EOC样本中检测到了CMV和EBV。结果提示HPV16可能是EOC发生的一个潜在危险因素。
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